cfKapture 21 (3-5 mL) - 50 preps

cfKapture™ 21 (3-5 mL)
Isolation kit for circulating cell-free DNA (cfDNA) from 3-5 mL stabilized plasma

The cfKapture™ 21 kit is a system for purification of circulating, cell-free nucleic acids from plasma. It is designed for the purification of circulating cell-free DNA(cfDNA) from maternal and cancer patient’s whole blood. The cfKapture 21 system includes all necessary reagents from the stabilization of separated plasma to the purification of cfDNA. The long term room temperature stabilization of plasma during storage or transport is achieved by mixing the plasma sample with our SE buffer allowing for up to 21-day room temperature stabilization of plasma samples. This functionality offers to samples processing laboratories, time flexibility for optimizing their operational efficiency as well as outreach for receiving samples at local and international levels. The isolated cfDNA can be directly used for real time-PCR and DNA library preparation suitable for next generation sequencing.

Features:

• cfDNA purification containing our proprietary plasma stabilization buffer.  (SE BUFFER)
• During purification process, separated plasma can be stabilized in our SE Buffer for 21 days.
• No Carrier RNA utilized.
• Not necessary to rush plasma purification. Large volume users can separate plasma and process samples without plasma sample degradation.
• Can be used with any blood collection tubes (Streck BCT, PAXGene ccfDNA tubes, EDTA).  For best results, use in conjunction with Blood STASIS 21-ccfDNA tubes.
   

Required Equipment:
15 mL X 50 mL combo magnetic separation rack 3X3


                                                                           
   

Work Flow


 

cfDNA purified from 400uL of plasma using MagBio's cfKapture 21, and QIAamp Circulating Nucleic Acid Kit. Plasma samples were separated from 1mL healthy male donor whole blood collected in EDTA,  Blood STASIS 21, PAXgene ccfDNA, and Streck BCT tubes store Room temperature for 1h. Yield was assessed by Qubit.

Note. Carrier RNA recommended by the manufacturer of QIAamp Circulating Nucleic Acid Kit was purposely not added during the extraction procedure to avoid skewed outcome numbers

Protocol
cfKapture 21 (3-5mL) protocol R1.2

Required Equipment
15 mL X 50 mL combo magnetic separation rack 3X3

MagStrip Magnet Stand (1.5mL x 10)

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