cfKapture™ 21 (3-5 mL)
Isolation kit for circulating cell-free DNA (cfDNA) from 3-5 mL stabilized plasma
The cfKapture™ 21 kit is a system for purification of circulating, cell-free nucleic acids from plasma. It is designed for the purification of circulating cell-free DNA(cfDNA) from maternal and cancer patient’s whole blood. The cfKapture 21 system includes all necessary reagents from the stabilization of separated plasma to the purification of cfDNA. The long term room temperature stabilization of plasma during storage or transport is achieved by mixing the plasma sample with our SE buffer allowing for up to 21-day room temperature stabilization of plasma samples. This functionality offers to samples processing laboratories, time flexibility for optimizing their operational efficiency as well as outreach for receiving samples at local and international levels. The isolated cfDNA can be directly used for real time-PCR and DNA library preparation suitable for next generation sequencing.
Features:
Required Equipment:
15 mL X 50 mL combo magnetic separation rack 3X3
Work Flow
cfDNA purified from 400uL of plasma using MagBio's cfKapture 21, and QIAamp Circulating Nucleic Acid Kit. Plasma samples were separated from 1mL healthy male donor whole blood collected in EDTA, Blood STASIS 21, PAXgene ccfDNA, and Streck BCT tubes store Room temperature for 1h. Yield was assessed by Qubit.
Note. Carrier RNA recommended by the manufacturer of QIAamp Circulating Nucleic Acid Kit was purposely not added during the extraction procedure to avoid skewed outcome numbers
Protocol
cfKapture 21 (3-5mL) protocol R1.2
Required Equipment
15 mL X 50 mL combo magnetic separation rack 3X3
Packaging | |
Volume Size | 50 Preps |
Storage and Stability | |
Storage | Store MAG-CFB Particles, Pro K Solution, DF Solution at 2-8°C | DO NOT FREEZE. Store all other components 15-25°C |
Stability | Stable for 12 months when all components are stored correctly. |
Disclaimers | |
Use Restriction | For Research Use Only. Not for Use in Diagnostic Procedures. |