Blood STASIS 21-ccfDNA blood collection tube

Blood STASIS™ 21 - ccfDNA
For collection of whole blood samples and stabilization of circulating cell-free DNA (ccfDNA) for up to 21 days
 

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The Blood STASIS™ 21-ccfDNA is a plastic, evacuated tube used for collection , anti-coagulation, stabilization, transport, and storage of venous whole blood as well as the preservation of circulating, cell-free DNA (ccfDNA).

  • Room temperature sample transport and storage for up to 21 days
  • Stabilization of circulating cell-free DNA (ccfDNA)
  • Direct draw blood tube for collection, stabilization, transport and storage
 
Blood STASIS 21-ccfDNA stabilization helps prevent apoptosis of blood cells and degradation of blood genomic DNA.
Whole blood samples were collected in the Blood STASIS 21-ccfDNA tube and K2EDTA tube; then incubated for 21 days at room temperature. Genomic DNA was extracted using MagBio HighPrep Blood & Tissue DNA Kit.  Extracted gDNA were analyzed using 1.2% agarose gel electrophoresis.
 
   
 
Plasma DNA was extracted from 1mL plasma from healthy control using MagBio
CF-Kapture 21 Kit. Four time points were tested (0, 7,14 and 21 days). Extracted plasma DNA was analyzed using Qubit and qPCR with Alu gene-specific primer sets (115bp; ALU 115) and long (247 bp, ALU 245) amplicons from genomic ALU repeats.  ccfDNA  shows small size distribution around 165bp; ALU 115-qPCR results accurately detect  the total quantity of ccfDNA and high molecular weight cellular genomic DNA. Alu247-qPCR results indicate only presence of high molecular weight cellular gDNA  contamination. The ratio ALU247/ALU115 is, therefore, an accurate indication ccfDNA integrity of the sample- T.B. hao in the British Journal of Cancer (2014) 111, 1482-1489). According to results from Hao and al. ccfDNA integrity indices are as follow:
 
 

 

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Blood STASIS 21-ccfDNA 9mL (100)
Blood STASIS™ 21 - ccfDNA For collection of whole blood samples and stabilization of circulatin..
Blood STASIS 21-ccfDNA 6mL (100)
Blood STASIS™ 21 - ccfDNA For collection of whole blood samples and stabilization of circulatin..
Blood STASIS 21-ccfDNA 3mL (200)
Blood STASIS™ 21 - ccfDNA For collection of whole blood samples and stabilization of circulatin..