FFPE DNA Kit

HighPrep™ FFPE Tissue DNA Kit
Xylene-Free deparaffinization.  No xylene/organic solvent treatment required.  Process safer and easier.

The Highprep™ FFPE Tissue DNA kit s a high quality, high throughput genomic purification of DNA from formalin-fixed, paraffin-embedded (FFPE) tissue samples utilizing magnetic beads.  The kit utilizes our proprietary non-organic deparaffinization buffer thus no xylene is required for safer processing and ease of use.  To overcome crosslinking of nucleic acids caused by formalin fixation, the kit uses specilally formulated buffers and lysing conditions to release DNA from tissue sections. Utilizing magnetic bead purification technology, the kit is high-throughput capable and can be adaped to most liquid handling workstations in the market.

Downstream Applications:

  • PCR
  • Sequencing
  • Sequencing (Next Generation)
  • Southern Blotting
  • Microarray Analysis
  • RT-PCR (Endpoint)
  • Real-Time PCR
  • Northern Blotting
  • cDNA Libray Construction

  
  DNA Yield and Purity

 

DNA Quality

Ct values of recovered DNA using the HighPrep FFPE Tissue DNA Kit for FFPE DNA purification. 
A 10-fold dilution series of the recovered DNA was used in a SYBR Green-based real-time PCR reaction targeting am 80-bp fragment of the human ribosomal protein gene RPL13a.  Each reaction was performed in triplicate. The input DNA amount were 100, 10, 1, 0.1 and 0.01 ng.

 

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HighPrep™ FFPE Tissue DNA Kit - Xylene-Free deparaffinization.  No xylene/organic solvent ..
HighPrep™ FFPE Tissue DNA Kit - Xylene-Free deparaffinization.  No xylene/organic solvent ..