HighPrep™ FFPE Tissue DNA Kit (96 Preps)

Product Code: HPFF-D96
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HighPrep™ FFPE Tissue DNA Kit
- Xylene-Free deparaffinization.  No xylene/organic solvent treatment required.  Process safer and easier with high quality yield.

The Highprep™ FFPE Tissue DNA kit is a high quality, high throughput genomic purification of DNA from formalin-fixed, paraffin-embedded (FFPE) tissue samples utilizing magnetic beads.  The kit utilizes our proprietary non-organic deparaffinization buffer thus no xylene is required for safer processing and ease of use.  To overcome crosslinking of nucleic acids caused by formalin fixation, the kit uses specilally formulated buffers and lysing conditions to release DNA from tissue sections. Utilizing magnetic bead purification technology, the kit is high-throughput capable and can be adaped to most liquid handling workstations in the market.

Downstream Applications:

  • PCR
  • Sequencing
  • Sequencing (Next Generation)
  • Southern Blotting
  • Microarray Analysis
  • RT-PCR (Endpoint)
  • Real-Time PCR
  • Northern Blotting
  • cDNA Libray Construction

  
  DNA Yield and Purity

 

DNA Quality

Ct values of recovered DNA using the HighPrep FFPE Tissue DNA Kit for FFPE DNA purification. 
A 10-fold dilution series of the recovered DNA was used in a SYBR Green-based real-time PCR reaction targeting am 80-bp fragment of the human ribosomal protein gene RPL13a.  Each reaction was performed in triplicates. The input DNA amount were 100, 10, 1, 0.1 and 0.01 ng.


Quality of DNA fragments after restoration following DNA extraction from two FFPE tissues using Magbio Genomic FFPE DNA Kit and Promega Maxwell 16 FFPE Tissue LEV DNA Kit

 

Packaging
Kit Components FFPE DP Reagent, FFPE DTL Buffer, FFPE DB Buffer, FFPE DW1 Buffer, FFPE DW2 Buffer, LPA, Elution Buffer, Pro K Solution, MAG-D3 Particles
Storage and Stability
Storage Store LPA, MAG-D3 particles 2-8°C. Store all other components 15-25°C. Pro K Solution is stable for 1 year when stored at 15-25°C. For storage longer than 1 year, store Pro K Solution at 2-8°C.
Stability 12 months when kit components are stored as directed.
Disclaimers
Use Restriction For Research Use Only. Not for Use in Diagnostic Procedures.
Number of Reactions - 96 Well Format
Preps 96

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Tags: ffpe dna