BigDye ® Sequencing Clean Up

HighPrep™ DTR
BigDye Terminator Removal Sequencing Clean Up Kit

HighPrep DTR is a high performance paramagnetic bead-based system for Big Dye sequencing clean up. Designed to remove unincorporated dye terminators from Sanger sequencing reaction, the HighPrep DTR system consists of a selective binding of DNA to the HighPrep DTR paramagnetic beads, followed with washing off nucleotides, primers and non-targeted amplicons, and finally elution of pure DNA. HighPrep DTR is designed for both manual and fully automated purification of sequencing products. The protocol can be adapted to your current liquid handling workstation (e.g. Beckman, Hamilton, Tecan, Perkin Elmer, Agilent and Eppendorf) utilizing your current protocol.

Features & Benefits

  • Long Phred 20 read lengths averaging over 800 bps
  • Pass rates over 85% or higher
  • Efficient elimination of sequencing reaction contaminants
  • Reduce BigDye usage

*BigDye is a registered trademark of Applied Biosystems

Applications

  • Clean up of sequencing product for both
    ABI and MegaBACE platforms

 

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HighPrep™ DTR (5 mL)
HighPrep DTR is a high performance paramagnetic bead-based system, designed to remove unincorpora..
HighPrep™ DTR (50 mL)
HighPrep DTR is a high performance paramagnetic bead-based system, designed to remove unincorpora..
HighPrep™ DTR (500 mL)
HighPrep DTR is a high performance paramagnetic bead-based system, designed to remove unincorpora..