The MagQuant Plus DNA Kit utilizes paramagnetic bead-based purification system for genomic DNA quantitation and concentration normalization. The DNA normalization occurs during the purification process. Standard methods of DNA quantitation require the use of a standard curve derived from DNA samples of known concentrations; processing DNA samples from various sources would require extensive work and reagent use. MagQuant DNA proprietary technology allows to obtain a given DNA quantitation and concentration from DNA samples regardless of the source by adding a specific amount of MagQuant DNA beads to the DNA sample.
MagQuant DNA Kit system eliminates the need for standard curve, and allowing the processing of a pool DNA samples with various source, thus leading to reducing labor and overall cost. The normalization process consists of binding genomic DNA to magnetic beads, then separating them on magnet, follow with washing of beads with water to remove excessive DNA, nucleotides, salts, and other contaminants, and finally eluting of the DNA. The process can be automated for high throughput applications on most of automated platforms currently on the market.
Applications:
Processing Time: ~40 min.
Protocols
MagQuant Plus DNA Protocol
Packaging | |
Kit Components | MAG-L1 Particles, SWB Buffer, NB Buffer, MB Elution Buffer, MB Wash Buffer |
Storage and Stability | |
Storage | MAG-L1 particles should be stored at 2-8°C. All other components stored at 15-25°C. |
Stability | Stable for 14 months when individual reagents stored at appropriate temperatures |
Disclaimers | |
Use Restriction | For Research Use Only. Not for Use in Diagnostic Procedures. |
Number of Reactions - 96 Well Format | |
Preps | 96 |