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HighPrep™ PCR (50 mL)
HighPrep™ PCR reagent is a post PCR and Next Gen library prep clean up system based on paramagnetic beads technology, designed for an efficient purification of PCR amplicons. The purification consists of removal of salts, primers, primer-dimers, dNTPs, as DNA fragments are selectively bound to the magnetic beads particles; and highly purified DNA is eluted with low salt elution buffer or water and can be used directly for downstream applications. The protocol can be adapted to your current liquid handling workstation (e.g. Beckman, Hamilton, Tecan, Caliper, Perkin Elmer, Agilent and Eppendorf) utilizing your current protocol as well as it can be performed manually.
Features:
- High recovery of amplicons greater than 100 bp
- Efficiently removes unincorporated dNTPs, primers, primer dimers and other contaminants
- Stable and high recovery of PCR products post-cleanup
- No centrifugation or filtration
Amplicons purified with HighPrep PCR system are ready to be used in the following applications:
- PCR
- Mutation detection and genotyping
- Sequencing (Sanger and Next Generation)
- Microarrays
- Restriction enzyme clean up
- Cloning
- Primer walking
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High Recovery of Amplicons Figure shows PCR products over 50 bp recovered from a 10µl PCR reaction. Data were analzyed on an Agilent Tapestation 2200. |
Protocols
HighPrep™ PCR Protocol
MSDS
HighPrep™ PCR - MSDS
Publications
Lars Velten, Simon Anders, Aleksandra Pekowska, Aino I Järvelin, Wolfgang Huber, Vicent Pelechano and Lars M Steinmetz
B. Divya Bhanu, Kandasamy Ulaganathan, Arun K. Shanker and S. Desai
Anna-Karin Berglund ,Clara Navarrete ,Martin K. M. Engqvist, Emily Hoberg, Zsolt Szilagyi, Robert W. Taylor, Claes, M. Gustafsson, Maria Falkenberg, Anders R. Clausen
Mark Livingstone
Joshua Quick
Mettu Madhavi Reddy, Kandasamy Ulaganathan
Rajendran Mathan Kumar,Gurwinder Kaur, Anand Kumar, Monu Bala, Nitin Kumar Singh, Navjot Kaur, Narender Kumar and Shanmugam Mayilraj
Ramesan Girish Nair, Gurwinder Kaur, Indu Khatri, Nitin Kumar Singh, Sudeep Kumar Maurya, Srikrishna Subramanian, Arunanshu Behera, Divya Dahiya, Javed N Agrewala and Shanmugam Mayilraj
Mettu M. Reddy and Kandasamy Ulaganathan
Martin Frederik Laursen, Gitte Zachariassen, Martin Iain Bahl, Anders Bergström, Arne Høst, Kim F. Michaelsen and Tine Rask Licht
Martin Frederik Laursen, Louise B. B. Andersen, Kim F. Michaelsen, Christian Mølgaard, Ellen Trolle, Martin Iain Bahl, Tine Rask Licht
Awanti Sambarey, Abhinandan Devaprasad, Abhilash Mohan, Asma Ahmed, Soumya Nayak, Soumya Swaminathan, George D’Souza, Anto Jesuraj, Chirag Dhar, Subash Babu, Annapurna Vykarnam, Nagasuma Chandra
Tiffany Leidy-Davis , Kai Cheng, Leslie O. Goodwin, Judith L. Morgan, Wen Chun Juan, Xavier Roca, Sin-Tiong Ong, David E. Bergstrom
Erick Strauss, Cynthia Kinsland, Ying Ge, Fred W. McLafferty, and Tadhg P. Begley
Franz Hölker, Christian Wurzbacher, Carsten Weißenborn, Michael T. Monaghan, Stephanie I. J. Holzhauer, Katrin Premke
• Novel Molecular Findings in Protein Tyrosine Phosphatase Receptor Gamma (PTPRG) Among Chronic Myelocytic Leukemia (CML) Patients Studied By Next Generation Sequencing (NGS): A Pilot Study in Patients from the State of Qatar and Italy
Maria Monne, Mohammed Araby, Ali Sayab, Marzia Vezzalini, Luisa Tomasello, Helmout Modjtahedi.
Ruibin Kuang, Kam-Ho Chan, Edward Yeung and Boon Leong Lim
Samet Azman & Ahmad F. Khadem & Caroline M. Plugge & Alfons J. M. Stams
De Tender, Lisa I. Devriese, Annelies Haegeman, Sara Maes, Tom Ruttink, and Peter Dawyndt
Rajendran Mathan Kumar, Gurwinder Kaur, Narender Kumar, Anand Kumar, Nitin Kumar Singh, Monu Bala, Navjot Kaur, Shanmugam Mayilraj
Lankelma, J.M.
Rajendran Mathan Kumar, Gurwinder Kaur, Narender Kumar, Anand Kumar, Nitin Kumar Singh, Monu Bala, Navjot Kaur and Shanmugam Mayilraj.
Anders R Clausen, Scott A Lujan, Adam B Burkholder, Clinton D Orebaugh, Jessica S Williams, Maryam F Clausen, Ewa P Malc, Piotr A Mieczkowski, David C Fargo, Duncan J Smith & Thomas A Kunkel
Sujay Paul, Yolanda Cortez, Nadia Vera, Gretty K. Villena, Marcel Gutiérrez-Correa
Yesupatham Sathishkumar, Chandran Krishnaraj, Kalyanaraman Rajagopa, Dwaipayan Sen and Yang Soo Lee
Peer H. A. Timmers, H. C. A. Widjaja-Greefkes, Javier Ramiro-Garcia, Caroline M. Plugge and Alfons J. M. Stams
Adriaan A. van Beek, Floor Hugenholtz, Ben Meijer, Bruno Sovran, Olaf Perdijk, Wilbert P. Vermeij, Renata M. C. Brandt, Sander Barnhoorn, Jan H. J. Hoeijmakers, Paul de Vos, Pieter J. M. Leenen, Rudi W. Hendriks and Huub F. J. Savelkoul.
Martin Frederik Laursen, Louise B. B. Andersen, Kim F. Michaelsen, Christian Mølgaard, Ellen Trolle, Martin Iain Bahl, Tine Rask Licht
Karen A. Cameron, Marek Stibal, Jakub D. Zarsky, Erkin Gozdereliler, Morten Schostag and Carsten S. Jacobsen
Mounir, Z., J. M. Korn, T. Westerling, F. Lin, C. A. Kirby, M. Schirle, G. McAllister
Fransen, Floris; Sahasrabudhe, Neha; Elderman, Marlies; Bosveld, Margaret; El Aidy, Sahar Farouk Abdelsalam; Hugenholtz, Floor; Borghuis, Theo; Kousemaker, Ben; Winkel, Simon; van der Gaast-de Jongh
Marcelle J. van der Waals, Siavash Atashgahi, Ulisses Nunes da Rocha, Bas M. van der Zaan, Hauke Smidt, Jan Gerritse
• Taxonomic description and genome sequence of Salinicoccus sediminis sp. nov., a halotolerant bacterium isolated from marine sediment
Rajendran Mathan Kumar, Gurwinder Kaur, Narender Kumar, Anand Kumar, Nitin Kumar Singh, Monu Bala, Navjot Kaur, Shanmugam Mayilraj
| Packaging | |
| Volume Size | 50 mL |
| Storage and Stability | |
| Storage | Store at 4°C | DO NOT FREEZE |
| Stability | Stable for 14 months when stored at 4°C |
| Disclaimers | |
| Use Restriction | For Research Use Only. Not for Use in Diagnostic Procedures. |
| Number of Reactions - 96 Well Format | |
| 10 µL PCR Reaction Volume | 2,778 |
| 20 µL PCR Reaction Volume | 1,389 |
| 50 µL PCR Reaction Volume | 556 |
| Number of Reactions - 384 Well Format | |
| 5 µL PCR Reaction Volume | 5,556 |
| 7 µL PCR Reaction Volume | 3,968 |
| 10 µL PCR Reaction Volume | 2,778 |
| 14 µL PCR Reaction Volume | 2,000 |
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