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cfKapture Kit
Magnetic bead-based DNA extraction kit designed to isolate circulating cell free DNA (cfDNA) from human plasma and serum.
Applications
cfDNA (ccfDNA) extraction for:
- Liquid biopsy research
- NIPT genomic research
- Cancer genomic research
- Transplant monitoring
Benefits
- Efficient Isolation of nucleic acids, with high input and low elution volumes
- No carrier RNA
- Low sample volume processing
- Efficient recovery of small sized fragmented DNA
- No organic extraction or ethanol precipitation
The cfKapture™ kit is a magnetic bead-based DNA extraction kit designed to isolate circulatingcell freeDNA (cfDNA) from human plasma and serum. The kit has a quick, simple, and automation friendly protocol that allows users to quickly extract high quality cfDNA that is ready for use in downstream applications such as qPCR, NGS, and bisulfite sequencing.
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cfKapture™ Kit process overview
This is a short overview of the protocol, for detailed protocols, please refer to PRODUCT DOCUMENTS
- Transfer plasma to a conical tube.
- Add Pro K Solution
- Add CFL Buffer
- Add 100% ethanol and MAG-CFB Particles
- Wash by adding CFW1 Buffer
- Wash by adding CFW2 Buffer
- Elution done by using Elution Buffer
- Store DNA at -20°C.
| Main functions | Isolation of cell free DNA (cfDNA) from plasma and serum |
| Starting material (Sample matrix) | Serum/plasma |
| Sample amount (Input) | 400 uL to 5000 uL |
| Process method | Manual or automation |
| Instrument | Adaptable to most nucleic acid purification instruments but a ready-made script for Kingfisher Flex is available upon request |
| Purification method | Magnetic bead-based technology |
| Elution Volume | 30-50 µl |
| Application | Next generation sequencing, Real time PCR and PCR |