cfKapture™ Kit
Magnetic bead-based DNA extraction kit designed to isolate circulating cell free DNA (cfDNA) from human plasma and serum.
Applications
cfDNA (ccfDNA) extraction for:
- Liquid biopsy research
- NIPT genomic research
- Cancer genomic research
- Transplant monitoring
Benefits
- Efficient Isolation of nucleic acids, with high input and low elution volumes
- No carrier RNA
- Low sample volume processing
- Efficient recovery of small sized fragmented DNA
- No organic extraction or ethanol precipitation
The cfKapture™ kit is a magnetic bead-based DNA extraction kit designed to isolate circulatingcell freeDNA (cfDNA) from human plasma and serum. The kit has a quick, simple, and automation friendly protocol that allows users to quickly extract high quality cfDNA that is ready for use in downstream applications such as qPCR, NGS, and bisulfite sequencing.
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cfKapture™ Kit process overview
This is a short overview of the protocol, for detailed protocols, please refer to PRODUCT DOCUMENTS
- Transfer plasma to a conical tube.
- Add Pro K Solution
- Add CFL Buffer
- Add 100% ethanol and MAG-CFB Particles
- Wash by adding CFW1 Buffer
- Wash by adding CFW2 Buffer
- Elution done by using Elution Buffer
- Store DNA at -20°C.
Kit Components
- CFL Buffer
- CFW1 Buffer
- CFW2 Buffer
- Elution Buffer
- Pro K Solution
- MAG-CFB Particles
Storage
Store MAG-CFB Particles, Pro K Solution at 2-8°C | DO NOT FREEZE. Store all other components 15-25°C.
Stability
Stable for 14 months when all components are stored correctly.
Disclaimers
For Research Use Only. Not for Use in Diagnostic Procedures.