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GENSeq™ DNA Library Preparation Kit
Magnetic beads based DNA Library Prep Kit for Illumina platforms
- Whole Genome Sequencing (Microbial DNA, Plant, Animal, and Human genomic DNA)
- Target Sequencing (FFPE DNA)
- High yields
- Reproducible
- Accurate
- Low GC bias
- On Target (over 99%)
- Low Input requirement (1ng lower limit)
- Minimized adaptor-dimer contamination
GENSeq™ DNA Library Prep Kit is designed for Illumina platforms; It is based on A-T ligation to prepare a library with high complexity for next -generation sequencing (NGS) from genomic DNA. The kit offers the best product stability, convenience and efficiency. It is suitable for library construction to perform whole genome and hybridization capture sequencing.
GENSeq™ DNA Library Prep Kit Workflow:
GENSeq DNA Library Prep Kit Performance Data:
GC BIAS:
YIELD:
REPRODUCIBILITY:
Kit Components:
GSDL-1024 GENSeq™ DNA Library Prep Kit (with adapters) (24 preps)
GSDL-1048 GENSeq™ DNA Library Prep Kit (with adapters) (48 preps) include 24 preps x2 GENSeq™ DNA Library Prep Kit (with adapters).
GSDL-1096 GENSeq™ DNA Library Prep Kit (with adapters) (96 preps) include 24 preps x4 GENSeq™ DNA Library Prep Kit (with adapters).
| Components | Shipping | Storage |
| End Repair & A-Tailing Buffer | -20 ℃ | -20 ℃ |
| End Repair & A-Tailing Enzyme | -20 ℃ | -20 ℃ |
| Ligation Buffer | -20 ℃ | -20 ℃ |
| DNA Ligase | -20 ℃ | -20 ℃ |
| 2X HiFi PCR Master Mix | -20 ℃ | -20 ℃ |
| Amplification Primer Mix | -20 ℃ | -20 ℃ |
| Nuclease Free Water | -20 ℃ | -20 ℃ |
| TE Solution | -20 ℃ | -20 ℃ |
| Adapters | -20 ℃ | -20 ℃ |
| Adapters | -20 ℃ | -20 ℃ |
| Magnetic Beads | Ambiemt Temp. | 4 ℃ |
Sample Quantity:1-1000 ng; By Qubit method
Kit Requirement: Sample Quality: gDNA without degradation by gel electrophoresis analysis; Size of FFPE gDNA >500 bp
Sample Purity: OD 260/280=1.8-2.0; OD 260/230=2-2.5
Ambient Temperature：20-25 ℃
Ambient Humidity：40 %-60 %
Disclaimers: For Research Use Only. Not for Use in Diagnostic Procedures.
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