VIRAL RNA EXTRACTION FOR COVID-19

Featured Products

HighPrep PCR

Magnetic bead-based reagent for manual and automated post-PCR and post-enzymatic reaction cleanup, NGS library cleanup, and reliable DNA size selection.

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HighPrep Viral DNA & RNA Kit

Magnetic bead based kit optimized for extraction of RNA from a variety of viral samples - Swabs, whole blood, serum, plasma, saliva & other body fluids.

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MagQuant Plus DNA Kit V2

Magnetic beads based kit for normalization of DNA concentration, and quantitation of DNA for NGS and other applications

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HighPrep Total RNA Plus Kit

Magnetic bead-based kit designed to extract high-quality total RNA from mammalian tissues and cultured cells.

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MagBio offers free samples of all kits & reagents for testing so that you can validate our products and ensure they suit your workflow before committing to any purchases.

Click on the link below or give us a call at (301) 302-0144 to request your free sample today!

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Latest News

. June 6, 2024

MagBio attends the ASM Microbe 2024 in Atlanta!

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. May 20, 2024

MagBio Launches the new Long-read sequencing extration kit

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. June 4, 2024

MagBio's new Long-read sequencing cleanup kit

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HighPrep PCR PB

HighPrep PCR PB is a paramagnetic bead-based product, specifically designed for SMRT sequencing workflow. It is used for DNA cleanup and size selection of SMRTbell® libraries. 

 Performance Data |  Comparative Data  |  Request a FREE Sample 


Grouped product items
Cat.# Product Name Price Qty
PB-60005 HighPrep PCR PB Kit (5mL)
$204.00
PB-60050 HighPrep PCR PB Kit (50mL)
$1,020.00
Applications

HighPrep PCR PB is suitable for PacBio applications such as:

  • SMRTbell® libraries clean-up and size selection

Benefits

  • Enhanced direct replacement of AMPure PB, no protocol

  • Cost effective. Save at least 40% or more when compared with the competitor PB

  • Amenable to automation (Kingfisher™ Flex script ready)

  • Purifies starting DNA material with low purity (low A260/A230) to remove contaminants

  • Efficiently removes unincorporated dNTPS, primer dimers, excess primers, salts, and other impurities from amplicons

  • Concentration of DNA from highly diluted samples

  • Depletes DNA fragmentsless than 5 kb

HighPrep PCR PB is a paramagnetic bead-based kit specifically designed for SMRT sequencing workflow. It is used for DNA cleanup and size selection of SMRTbell® libraries. 

HighPrep PCR PB is free of reagents that can interfere with polymerase activity during sequencing, therefore, templates bind properly to the polymerase. This product is designed for both manual and fully automated purification of DNA and sequencing products.

This is a short overview of the protocol. For a detailed protocol, please refer to PRODUCT DOCUMENTS.

DNA Clean-up/Purification

  1. Add 0.45x HighPrep PCR PB reagent to the sample
  2. Bind DNA to paramagnetic beads
  3. Separation of beads from the supernatant with contaminants
  4. Wash beads with Ethanol to remove contaminants
  5. Elute purified DNA from beads                                          

 Fragment Selection

  1. Dilute HighPrep PCR PB to 35% v/v
  2. Add HighPrep PCR PB reagent (based on desired fragment size) to the sample
  3. Bind DNA to paramagnetic beads
  4. Separation of beads from the supernatant with contaminants
  5. Wash beads with Ethanol to remove contaminants
  6. Elute purified DNA from beads

Nucleic Acid Input

Fragmented DNA, NGS and TGS libraries, PCR products

Starting Amount

Scalable

Recovery

>80%

Process Method

Automated; Manual

Instrument

Adaptable to most nucleic acid purification instruments, but a ready-made script for Kingfisher™ Flex is available upon request

Elution Volume

10 uL or above

Cleanup

Removes unwanted reaction components to purify DNA

Size Selection

Adjustable bead ratio based on desired fragment size

Automated Extraction on Kingfisher Flex

45 minutes

Downstream Applications

Genomic DNA cleanup, NGS library cleanup, Size selection, PCR, SMRTbell® libraries clean-up and size selection