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HighPrep Viral-Bacterial DNA & RNA Kit
Magnetic beads based kit for rapid isolation of viral and bacterial nucleic acids from whole blood, serum, plasma, saliva and other body fluids.
Sensitivity Data | Comparative Data | VTM COMPATIBILITY
Viral/Bacterial RNA and DNA isolation for:
- RT-qPCR; RT-PCR, PCR
- One-Step RT-qPCR
- Virus detection, genotyping
- Viral load monitoring,
- OPTIMIZED FOR ISOLATION from BACTERIAL & VIRAL samples.
- Rapid and reliable purification of nucleic acids
- Adaptable to various automated liquid handling workstations
- No toxic organic solvents
The HighPrep Viral-Bacterial DNA & RNA kit is designed for rapid and reliable isolation of viral & bacterial nucleic acids from whole blood, serum, plasma, saliva and other body fluids as well as nasopharyngeal swabs soaked in virus transport media or other buffers. This kit is highly efficient in vIral nucleic acid isolation and the extracted RNA (and DNA) is suitable for direct use in most downstream applications such as one-step RT-qPCR, RT-PCR, PCR, nucleic acid amplification, cloning, sequencing, and enzymatic reactions. The kit can be used in low throughput manual workflows and is also adaptable to majority of the liquid handling workstations in the market.
This is a short overview of the protocol, for all detailed protocols, please refer to PRODUCT DOCUMENTS
- Add the sample fluid into the well or tube.
- Add Lysis Buffer, Proteinase K and incubate at 56-60°C to perform cell lysis.
- Add NBE, isopropanol (Not Supplied) and MAG-S1 particles. Incubate and bind to nucleic acids
- Perform one wash step with RDW Buffer (Mixed with 100% ethanol)
- Wash the beads 2 times with 70% ethanol (Not Supplied)
- Perform nucleic acid elution using supplied Nuclease-Free Water
- Store NA at -20°C
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Features |
Specifications |
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Main functions |
Isolation of Viral and Bacterial DNA/RNA from various sample matrices |
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Starting material (Sample matrix) |
Whole blood, saliva, urine, swabs, plasma, samples collected in Transport Media, serum |
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Sample amount (Input) |
can be scaled up |
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Downstream application |
Real time quantitative PCR, Reverse Transcriptase PCR, PCR, NGS |
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Process method |
Manual or automation |
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Instrument |
Adaptable to most nucleic acid purification instruments but a ready-made script for Kingfisher Flex is available upon request |
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Purification method |
Magnetic bead-based technology |
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Purification time |
23 min on Kingfisher™ Flex Instrument |
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