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HighPrep Viral-Pathogen DNA & RNA Kit
One kit and one standardized protocol enable multi-pathogen extraction across diverse sample types.
Magnetic bead–based kit for rapid, high-recovery isolation of viral, bacterial, and fungal nucleic acids from whole blood, serum, plasma, saliva, urine, swabs, and other body fluids, supporting sensitive detection even at low pathogen loads. It streamlines extraction workflows with one kit and one standardized protocol across multiple pathogens and a broad range of sample matrices. This kit is optimized for high-throughput pathogen detection workflows on KingFisher™ Flex and other automation platforms.
Viral/Pathogen RNA & DNA extraction for:
- RT-qPCR, RT-PCR, and PCR
- One-step RT-qPCR
- Viral and bacterial detection, genotyping, and variant surveillance
- Viral load and pathogen load monitoring
- NGS and other DNA/RNA-based workflows
- One kit & one standardized protocol
Extract DNA and RNA from viral, bacterial, and fungal pathogens across diverse sample matrices in a single workflow; simplifying training, validation, and method transfer. - High sensitivity at low pathogen loads
Supports sensitive recovery at low pathogen loads for early detection and greater confidence in results. - Robust inhibitor tolerance
Consistent performance in inhibitor-rich samples (urine, whole blood, wound exudate), minimizing failed and repeat runs. - Rapid & reliable purification
High-purity nucleic acids that amplify on the first run, reducing failed and repeat extractions. No toxic organic solvents. - Automation and time efficiency
Plug-and-run on KingFisher™ Flex (~52 minutes with a ready-to-use script) and adaptable to most liquid-handling workstations, reducing hands-on time, freeing staff, and stabilizing turnaround times. - Comparable performance with cost savings
Improved drop-in replacement for the MagMAX™ Viral/Pathogen Ultra Nucleic Acid Isolation Kit, delivering comparable performance at up to 20% lower cost per prep.
The HighPrep™ Viral-Pathogen DNA & RNA Kit is a paramagnetic bead-based kit designed for rapid isolation of viral, bacterial, and fungal nucleic acids from whole blood, serum, plasma, saliva, urine, and other body fluids, as well as nasopharyngeal swabs soaked in virus transport media or other compatible buffers. The upgraded chemistry delivers high-recovery, high-quality DNA and RNA that support sensitive detection even at low pathogen loads and consistent performance in inhibitor-rich samples. The purified nucleic acids are ready for direct use in downstream applications such as RT-qPCR, RT-PCR, PCR, NGS, and other DNA/RNA-based workflows. The kit supports both low-throughput manual protocols (using magnetic stands or racks) and high-throughput automated workflows on KingFisher™ Flex and other nucleic acid purification instruments. The same core chemistry is used across multi-pathogen workflows and diverse sample matrices to minimize re-validation, making it easy to scale from method development to routine testing while maintaining assay consistency.
This is a short overview of the protocol, for all detailed protocols, please refer to PRODUCT DOCUMENTS Section.
- Enzyme digestion: Digest microbial pathogens with LES I and LES II to promote efficient downstream lysis.
- Lysis: Lyse cells to release DNA and RNA into solution using Lysis Buffer and Proteinase K.
- DNA binding: Bind nucleic acids to MAG-S1 particles in the presence of 100% Isopropanol and a chaotropic salt. NBE enhances nucleic acid binding to the beads.
- Bead wash: Wash MAG-S1 particles with Wash Buffer followed by 80% Ethanol to remove contaminants.
- Nucleic acid elution: Elute nucleic acids by adding a low-molarity, mildly basic buffer, releasing DNA/RNA from the beads into Elution Buffer.
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Features |
Specifications |
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Main function |
One kit and one standardized protocol for isolation of multi-pathogen DNA/RNA from diverse sample matrices. |
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Starting material (sample matrix) |
Cell-free body fluids (serum, plasma, urine), whole blood, saliva, respiratory and other swabs, VTM, and other compatible body fluids |
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Sample amount (input) |
Flexible and scalable input volume; protocol can be scaled up according to assay requirements |
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Downstream applications |
Real-time quantitative PCR, Reverse Transcriptase PCR, PCR, NGS, and other DNA/RNA-based workflows |
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Process method |
Manual or automated processing |
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Instrumentation |
Adaptable to most nucleic acid purification instruments; ready-made script for KingFisher™ Flex available upon request |
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Purification method |
Magnetic bead–based technology |
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Purification time |
~52 minutes on KingFisher™ Flex Instrument |
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