HighPrep™ Blood DNA-FTA Kit

magnetic beads based kit for high quality genomic DNA isolation from blood samples stabilized on FTA cards


Grouped product items
Cat.# Product Name Price Qty
HPBS-DBS96 HighPrep™ Blood DNA-FTA Kit (96 Preps)
$265.17
HPBS-DBS96x4 HighPrep™ Blood DNA-FTA Kit (384 Preps)
$878.24
Applications

Genomic DNA extraction for:

  • PCR, Real-time PCR
  • Southern blotting
  • Cloning, genotyping
  • Sequencing

Benefits

  • Efficient isolation of DNA from FTA card
  • High yield of DNA from small volume blood samples
  • Less fragmented and much purer gDNA

HighPrep™ Blood DNA-FTA Kit utilizes paramagnetic beads to isolate genomic DNA (gDNA) from FTA card; resulting in high quantity and quality DNA from human blood spots usable in various genomic downstream applications (ie PCR, and qPCR). HighPrep™ Blood DNA-FTA Kit could be used for few samples as well as in high throughput setting on automated workflow.

This is a short overview of the protocol, for detailed protocols, please refer to PRODUCT DOCUMENTS
  1. Prepare Sample
  2. Lyse using MB Lysis Buffer and add Pro K Solution
  3. Add MB Binding Buffer
  4. Add 100% Ethanol and Mag-D2 Particles and let the DNA bind to the beads.
  5. Add MPW Buffer
  6. Add FDW Buffer for 2 washes
  7. Elute using heated Elution Buffer
  8. Store DNA at -20°C.
Kit Components

  • Mag-D2 Particles
  • MB Lysis Buffer
  • MB Binding Buffer
  • MPW Wash Buffer
  • FDW Wash Buffer
  • MB Elution Buffer
  • Proteinase K Solution
  • DNA Binding Enhancer DBE
Storage

MAG-D2 particles, Pro K solution and DNA Binding Enhancer should be stored at 2-8°C. All other components stored at 15-25°C.

Stability

14 months when kit components are stored as directed.

Disclaimers

For Research Use Only. Not for Use in Diagnostic Procedures.

No references yet. Want to share one? Write us an email.