HighPrep™ DTR Clean-up System

Magnetic beads based reagent for manual and automated Sanger sequencing reaction clean-up for both ABI and MegaBACE sequencing platforms.


Grouped product items
Cat.# Product Name Price Qty
DT-70005 HighPrep™ DTR (5 mL)
$156.98
DT-70050 HighPrep™ DTR (50 mL)
$1,054.31
DT-70250 HighPrep™ DTR (250 mL)
$3,606.31
DT-70500 HighPrep™ DTR (500 mL)
$6,300.43
Applications

Sanger sequencing reaction clean-up / dye terminator removal for:

  • Sanger sequencing on ABI platforms
  • Sanger sequencing on MegaBACE platforms

Benefits

  • Rapid and reliable clean-up of sequencing reactions
  • High DNA recovery yields, long sequence readings
  • Flexible - can be used in any format
  • No centrifugation step, no filtration step
  • Adaptable to common liquid handling workstations

The HighPrep™ DTR Clean-up System is a high performance paramagnetic bead-based reagent for BigDye™ terminator removal from Sanger sequencing reactions for reliable and long readings of DNA sequences. The HighPrep™ DTR Clean-up System consists of a selective binding of DNA to the paramagnetic beads, followed with washing off nucleotides, primers, and non-targeted amplicons, and finally elution of pure DNA. HighPrep™ DTR Clean-up System is an excellent choice for both manual and fully automated purification of sequencing reaction products. The protocol can be adapted to common liquid handling workstation.

BigDye is a registered trademark of Applied Biosystems

This is a short overview of the protocol, for all detailed protocols, please refer to PRODUCT DOCUMENTS
  1. Add HighPrep™DTR reagent to sample
  2. Add freshly prepared 85% ethanol
  3. Bind sample to paramagnetic beads
  4. Wash beads with 85% ethanol to remove contaminants
  5. Elute purified DNA from beads
Kit Components

  • HighPrep™ DTR Clean-up reagent
Storage

Store at 4°C to 8°C | DO NOT FREEZE

Stability

14 months when stored as directed.

Disclaimers

For Research Use Only. Not for Use in Diagnostic Procedures.

Number of reactions
In 96 Well Format
PCR Reaction Volume Number of reactions
5 µL 500
10 µL 500
15 µL 500
20 µL 500
In 384 Well Format
PCR Reaction Volume Number of reactions
5 µL 1000
10 µL 1000
15 µL 1000

  1. An economical and efficient method of post reaction cleanup of labeled dye terminator sequencing products. Rajesh R Kundapur, Vijay Nema
  2. Knock-In of a 25-Kilobase Pair BAC-Derived Donor Molecule 9 by Traditional and CRISPR/Cas9-Stimulated Homologous Recombination. Tiffany Leidy-Davis, Kai Cheng, Leslie O. Goodwin, Judith L. Morgan, Wen Chun Juan, Xavier Roca, Sin-Tiong Ong, David E. Bergstrom
  3. Nuclear introgression without mitochondrial introgression in two turtle species exhibiting sex-specific trophic differentiation. Sarah M. Mitchell,nLaura K. Muehlbauer, Steven Freedberg
  4. A European epidemiological survey of Vibrio splendidus clade shows unexplored diversity and massive exchange of virulence factors. Nasfi H, Travers MA, de Lorgeril J, Habib C, Sannie T, Sorieul L, Gerard J, Avarre JC, Haffner P, Tourbiez D, Renault T, Furones D, Roque A, Pruzzo C, Cheslett D, Gdoura R, Vallaeys T.
  5. Factors affecting Sanger sequencing robustness and data quality. Patrick Warner, Shea Anderson, Archana Deshpande, Daryl M. Gohl, Kenneth B. Beckman

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