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HighPrep PCR
Paramagnetic bead-based post PCR clean-up reagent designed for efficient DNA purification and consistent DNA fragment size selection for NGS. HighPrep PCR is adaptable to most liquid handling stations and used for applications such as sequencing, NGS, microarrays, PCR, and enzymatic reactions.
Applications
Post-PCR and post-enzymatic reaction clean-up used for/during:
- NGS library preparation
- DNA size selection for NGS
- Microarrays
- PCR
- Restriction enzyme digestions, adapter ligations
- Cloning
Benefits
- Rapid and reliable post-PCR and DNA clean-up reagent
- Achieve uniform and consistent DNA fragments
- High recovery of amplicons >100bp
- Uniform fragments size distribution
- Adaptable to high throughput liquid handling workstations
HighPrep PCR is a paramagnetic beads-based post PCR clean-up reagent designed for efficient purification of amplicons and tight size-specific selection of DNA fragments. The purification consists of removal of salts, primers, primer-dimers, dNTPs, as DNA fragments are selectively bound to the magnetic bead particles. Highly purified DNA is eluted with low salt elution buffer or water which can be used directly for downstream applications, such as library construction, next generation sequencing (NGS), Sanger sequencing, cloning, restriction digestions, adapter ligation, microarrays, and so on. This protocol can be used for manual procedures as well as in automation.
| Product information area | CE-IVD and UKCA Registered |
|---|
This is a short overview of the protocol, for all detailed protocols, please refer to PRODUCT DOCUMENTS
- Add HighPrep™ PCR reagent to the reaction mixture
- Bind DNA to paramagnetic beads
- Separation of beads + DNR from contaminants
- Wash beads with Ethanol to remove contaminants
- Elute purified PCR products or other DNA fragments from beads
| Main functions | Purifies DNA and amplicons for next generation sequencing and other applications Selectively binds fragments of 100 bp and larger depending on sample to bead ratio – performs size selection by changing the bead ratio |
| Starting material (Sample matrix) | DNA, PCR products, cDNA, gDNA |
| Starting amount | Scalable |
| RNA recovery | >70% recovery |
| Process method | Manual or automation |
| Instrument | Adaptable to most nucleic acid purification instruments but a ready-made script for Kingfisher Flex is available upon request |
| Purification method | Magnetic bead-based technology |
| RNA Purity | DNA eluted is ready for downstream processes such as real time quantitative PCR (qPCR), next generation sequencing (NGS), cloning, restriction digestions, microarrays, etc. |
| Elution Volume | 15 µl or above |
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