HighPrep Plant DNA Plus Kit
The HighPrep™ Plant DNA Plus Kit is specially designed for purifying DNA from a wide range of plant and fungi species. The kit uses the special lysis condition with HighPrep™ magnetic particles technology to isolate the high-quality genomic DNA. Under DNA binding conditions only the genomic DNA will bind to the magnetic particles while most of the contaminating cellular proteinaceous components are removed. The purifi ed DNA is of the highest integrity, and can be used in a number of downstream applications including real time PCR, Southern blotting, SNP analysis and NGS etc. The HighPrep™ Plant DNA Plus Kit (Magnetic Bead System) can be easily adapted to automated magnetic bead separation instruments and work stations.
This is a short overview of the protocol, for all detailed protocols, please refer to PRODUCT DOCUMENTS
- Plant samples are disrupted in a homogenizer/bead based milling equipment.
- PLB Buffer is added to lyse the sample.
- Supernatant is then transferred to a new processing plate where MAG-D1 particles are added to bind to the DNA.
- Following a few wash steps, DNA is eluted from the MAG-D1 particles for downstream application.
|Isolation of DNA from a wide range of plant and fungi species.
|Starting material (Sample matrix)
|Plant and fungi species including any pathogen DNA present
|Sample amount (Input)
|Can be scaled up
|Leaves, stems, flowers, seeds, fruits, etc.
|Manual or automation
|Need a disruptor/pulverizer for sample homogenization.
The DNA extraction protocol is adaptable to most nucleic acid purification instruments but a ready- made script for Kingfisher Flex is available upon request
|Magnetic bead-based technology
|≥ 50 µl
|Capable of recovering genomic DNA sized fragments > 50 kb. If present, parasitic, bacterial, and viral DNA can be recovered.
|High quality DNA is ready for all sensitive downstream applications such as Real time quantitative PCR, PCR, Next Generation Sequencing.
A260/A280 ratio of ≥1.8
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