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HighPrep PCR

Magnetic bead-based reagent for manual and automated post-PCR and post-enzymatic reaction cleanup, NGS library cleanup, and reliable DNA size selection.

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HighPrep Viral DNA & RNA Kit

Magnetic bead based kit optimized for extraction of RNA from a variety of viral samples - Swabs, whole blood, serum, plasma, saliva & other body fluids.

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MagQuant Plus DNA Kit V2

Magnetic beads based kit for normalization of DNA concentration, and quantitation of DNA for NGS and other applications

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HighPrep Total RNA Plus Kit

Magnetic bead-based kit designed to extract high-quality total RNA from mammalian tissues and cultured cells.

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HighPrep Plasmid DNA Plus Kit

Magnetic beads based kit designed to extract high-quality plasmid DNA (including Cosmids, Fosmids, and BACs) from bacterial cultures.

Grouped product items
Cat.# Product Name Price Qty
HPPL-D96 HighPrep Plasmid DNA Plus Kit (96 preps)
HPPL-D96x4 HighPrep Plasmid DNA Plus Kit (384 Preps)
HPPL-D96x20 HighPrep Plasmid DNA Plus Kit (1920 Preps)
HPPL-D96x40 HighPrep Plasmid DNA Plus Kit (3840 Preps)

Plasmid DNA isolation for:

  • PCR, Real-time PCR
  • Cloning, genotyping
  • Sequencing, NGS
  • Transfection


  • High quality plasmid DNA purification
  • High yields of both high and low copy number plasmids
  • Adaptable to various automated liquid handling workstations
  • No toxic organic solvents

The HighPrep™ Plasmid DNA kit is a magnetic beads-based plasmid purification system for high throughput rapid isolation of both high and low copy number plasmid DNA from bacterial cultures as well as for isolation of BACs, PACs, Cosmids or Fosmids.  The system can easily be automated on many liquid handling workstations, providing robustness and high-quality plasmid DNA for subsequent downstream applications such as restriction digestion, PCR, cloning and fluorescent DNA sequencing.

This is a short overview of the protocol, for all detailed protocols, please refer to PRODUCT DOCUMENTS
  1. Grow 1.0-1.5 ml E.coli LB cultures in a 2 mL 96-well culture plate at 37°C with agitation for 16-20 hours.
  2. Add SOL1 for suspension
  3. Add SOL2 for lysis
  4. Add of chilled (4°C) Neutralization Buffer
  5. Add MAG-S1 particles and 100% Isopropanol to each well.
  6. Wash the wells with 70% ethanol and mix
  7. Elution done using MB Elution Buffer
  8. Store DNA at -20°C.
Features Specifications
Main functions DNA isolation of low/high copy plasmid DNA, Cosmid and Fosmids
Starting material (Sample matrix) 1.0 to 1.5 mL of culture; Culture absorbance (OD600) should be between 2 and 3
Process method Manual or automation
Instrument Adaptable to most nucleic acid purification instruments but a ready-made script for Kingfisher Flex is available upon request
Purification method Magnetic bead-based technology
Elution Volume ≥ 50 µl
Purity Plasmid DNA can be used in a wide variety of downstream applications such as sequencing, PCR, expression of proteins, transfection, and gene
Lysate clearance Lysate cleared by centrifugation

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