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HighPrep RNA Elite
Magnetic beads based reagent for manual and automated clean-up or concentration of RNA (including miRNA, siRNA, aRNA) and single-stranded cDNA after enzymatic reactions or prior sequencing.
RNA and ss cDNA clean-up or concentration for or after:
- cDNA synthesis
- RT-PCR
- Sequencing
- In vitro transcription
- RNA probe synthesis
- miRNA and siRNA preparation
- Rapid and reliable clean-up of ss cDNA and RNA, including miRNA, siRNA, and aRNA
- Efficient purification of both large and small RNAs, and cDNA
- Complete removal of salts, unincorporated primers, and nucleotides
- No centrifugation step, no filtration step
- Adaptable to your current liquid handling workstation
The HighPrep RNA Elite utilizes MagBio’s solid-phase paramagnetic bead-based technology for high-throughput purification of RNA or cDNA for in vitro applications such as transcription, antisense RNA (aRNA) amplification as well as for RNA and cDNA probe synthesis. This protocol enables recovery even of micro RNA (miRNA), small RNA and total RNA from enzymatic reactions, concentrating of miRNA and total RNA from a diluted sample. The HighPrep RNA Elite can be used for manual RNA clean-up as well as on automated liquid handling workstations.
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HighPrep RNA Elite provides efficient RNA clean-up with the high recovery yield
This is a short overview of the protocol, for all detailed protocols, please refer to PRODUCT DOCUMENTS
- Add HighPrep™ RNA Elite reagent to sample
- Bind RNA to paramagnetic beads
- Separation of beads from contaminants
- Wash beads with 70% ethanol to remove contaminants
- Elute purified RNA or cDNA from beads
Kit Components
- HighPrep™ RNA Elite Clean-up reagent
Storage
Store at 4°C to 8°C | DO NOT FREEZE
Stability
14 months when stored as directed.
Disclaimers
Number of reactions
| In 96 Well Format | |
| PCR Reaction Volume | Number of reactions |
| 10 µL | 55 |
| 14 µL | 40 |
| In 384 Well Format | |
| PCR Reaction Volume | Number of reactions |
| 5 µL | 556 |
| 7 µL | 397 |
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