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HighPrep Total RNA Plus Kit
Magnetic bead-based kit designed to extract high-quality total RNA from mammalian tissues and cultured cells.
Applications
Total RNA isolation for:
- RT-qPCR
- RNA sequencing
- cDNA synthesis
- Gene expression analysis
Benefits
- High yield and quality total RNA purification
- Adaptable to various automated liquid handling workstations
- No toxic organic solvents
The HighPrep™ Total RNA Plus kit is a paramagnetic bead-based system that allows for a fast and efficient purification of high-quality total RNA from tissues and cultured cells suitable for numerous high-performance RNA downstream applications such as RT-PCR, microarrays, cDNA synthesis. As a paramagnetic bead-based system, the HighPrep™ Total RNA kit can be used in a high-throughput format, hence adaptable to numerous commercially available workstations.
This is a short overview of the protocol, for all detailed protocols, please refer to PRODUCT DOCUMENTS
- Prepare Sample and add Solution A
- Add Pro K Solution
- Add 100% Ethanol and Mag-D4 Particles and let the RNA bind to the beads
- Wash using MRW1 Buffer, RWB2 Buffer and DNase mix.
- Repeat wash steps
- Elution done using RNA Elution Buffer
- Store the RNA at -20°C.
| Features | Specifications |
| Main functions | Isolation of total RNA from various tissues, cultured cells, buccal swabs, and whole blood |
| Starting material (Sample matrix) | |
| Swab lysate, tissues, whole blood, and cultured cells |
|
| Sample amount (Input) | About 30 mg of fresh or preserved tissue, 200 ul of whole blood, 1 x 10^6 cells or 200 ul of swab lysate |
| Downstream application | PCR, RT-PCR, real-time qRT-PCR, microarray, Northern hybridization, next generation sequencing |
| Process method | Manual or automation |
| Instrument | Adaptable to most nucleic acid purification instruments but a ready-made script for Kingfisher Flex is available upon request |
| Purification method | Magnetic bead-based technology |
| RNA Purity | OD260/A280:1.9-2.0, OD260/230:1.7-2.0 |
| Elution Volume | ≥30 ul |
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