VIRAL RNA EXTRACTION FOR COVID-19

Featured Products

HighPrep PCR

Magnetic bead-based reagent for manual and automated post-PCR and post-enzymatic reaction cleanup, NGS library cleanup, and reliable DNA size selection.

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HighPrep Viral DNA & RNA Kit

Magnetic bead based kit optimized for extraction of RNA from a variety of viral samples - Swabs, whole blood, serum, plasma, saliva & other body fluids.

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MagQuant Plus DNA Kit V2

Magnetic beads based kit for normalization of DNA concentration, and quantitation of DNA for NGS and other applications

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HighPrep Total RNA Plus Kit

Magnetic bead-based kit designed to extract high-quality total RNA from mammalian tissues and cultured cells.

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Click on the link below or give us a call at (301) 302-0144 to request your free sample today!

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HighPrep Total RNA Plus Kit

Magnetic bead-based kit designed to extract high-quality total RNA from mammalian tissues and cultured cells.


Grouped product items
Cat.# Product Name Price Qty
HPTOR-R50 HighPrep Total RNA Plus Kit (50 Preps)
$349.43
HPTOR-R100 HighPrep Total RNA Plus Kit (100 Preps)
$652.69
HPTOR-R100x4 HighPrep Total RNA Plus Kit (400 Preps)
$1,958.07
Applications

Total RNA isolation for:

  • RT-qPCR
  • RNA sequencing
  • cDNA synthesis
  • Gene expression analysis

Benefits

  • High yield and quality total RNA purification
  • Adaptable to various automated liquid handling workstations
  • No toxic organic solvents

The HighPrep™ Total RNA Plus kit is a paramagnetic bead-based system that allows for a fast and efficient purification of high-quality total RNA from tissues and cultured cells suitable for numerous high-performance RNA downstream applications such as RT-PCR, microarrays, cDNA synthesis. As a paramagnetic bead-based system, the HighPrep™ Total RNA kit can be used in a high-throughput format, hence adaptable to numerous commercially available workstations.

This is a short overview of the protocol, for all detailed protocols, please refer to PRODUCT DOCUMENTS
  1. Prepare Sample and add Solution A
  2. Add Pro K Solution
  3. Add 100% Ethanol and Mag-D4 Particles and let the RNA bind to the beads
  4. Wash using MRW1 Buffer, RWB2 Buffer and DNase mix.
  5. Repeat wash steps
  6. Elution done using RNA Elution Buffer 
  7. Store the RNA at -20°C.
Features Specifications
Main functions Isolation of total RNA from various tissues, cultured cells, buccal swabs, and whole blood
Starting material (Sample matrix)
Swab lysate, tissues, whole blood, and cultured
cells
Sample amount (Input) About 30 mg of fresh or preserved tissue, 200 ul of whole blood, 1 x 10^6 cells or 200 ul of swab lysate
Downstream application PCR, RT-PCR, real-time qRT-PCR, microarray, Northern hybridization, next generation sequencing
Process method Manual or automation
Instrument Adaptable to most nucleic acid purification instruments but a ready-made script for Kingfisher Flex is available upon request
Purification method Magnetic bead-based technology
RNA Purity OD260/A280:1.9-2.0, OD260/230:1.7-2.0
Elution Volume ≥30 ul

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