HighPrep Total RNA Plus Kit
Magnetic bead-based kit designed to extract high-quality total RNA from mammalian tissues and cultured cells.
Total RNA isolation for:
- RNA sequencing
- cDNA synthesis
- Gene expression analysis
- High yield and quality total RNA purification
- Adaptable to various automated liquid handling workstations
- No toxic organic solvents
The HighPrep™ Total RNA Plus kit is a paramagnetic bead-based system that allows for a fast and efficient purification of high-quality total RNA from tissues and cultured cells suitable for numerous high-performance RNA downstream applications such as RT-PCR, microarrays, cDNA synthesis. As a paramagnetic bead-based system, the HighPrep™ Total RNA kit can be used in a high-throughput format, hence adaptable to numerous commercially available workstations.
This is a short overview of the protocol, for all detailed protocols, please refer to PRODUCT DOCUMENTS
- Prepare Sample and add Solution A
- Add Pro K Solution
- Add 100% Ethanol and Mag-D4 Particles and let the RNA bind to the beads
- Wash using MRW1 Buffer, RWB2 Buffer and DNase mix.
- Repeat wash steps
- Elution done using RNA Elution Buffer
- Store the RNA at -20°C.
|Main functions||Isolation of total RNA from various tissues, cultured cells, buccal swabs, and whole blood|
|Starting material (Sample matrix)|
|Swab lysate, tissues, whole blood, and cultured
|Sample amount (Input)||About 30 mg of fresh or preserved tissue, 200 ul of whole blood, 1 x 10^6 cells or 200 ul of swab lysate|
|Downstream application||PCR, RT-PCR, real-time qRT-PCR, microarray, Northern hybridization, next generation sequencing|
|Process method||Manual or automation|
|Instrument||Adaptable to most nucleic acid purification instruments but a ready-made script for Kingfisher Flex is available upon request|
|Purification method||Magnetic bead-based technology|
|RNA Purity||OD260/A280:1.9-2.0, OD260/230:1.7-2.0|
|Elution Volume||≥30 ul|