VIRAL RNA EXTRACTION FOR COVID-19

Featured Products

HighPrep™ PCR Clean-up System

Magnetic beads based reagent for manual and automated post-PCR and post-enzymatic reaction clean-up, for NGS library clean-up and reliable amplicon size selection.

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HighPrep™ Viral DNA/RNA Kit

Magnetic bead based kit optimized for extraction of RNA from a variety of viral samples - Swabs, whole blood, serum, plasma, saliva & other body fluids.

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MagQuant™ Plus DNA Kit

Magnetic beads based kit for normalization of DNA concentration, and quantitation of DNA for NGS and other applications

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HighPrep™ Total RNA Plus Kit

Magnetic bead-based kit designed to extract high-quality total RNA from mammalian tissues and cultured cells.

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HighPrep Total RNA Isolation Kit - iSWAB™

Magnetics bead-based kit designed to extract high-quality RNA from samples collected using the MAWI iSWAB V2 Collection Kit (ISWAB-RNA-v2)


Grouped product items
Cat.# Product Name Price Qty
ISTOR-R50 HighPrep Total RNA Isolation Kit - iSWAB™ (50 Preps)
$416.95
ISTOR-R100 HighPrep Total RNA Isolation Kit - iSWAB™ (100 Preps)
$692.01
ISTOR-R100x4 HighPrep Total RNA Isolation Kit - iSWAB™ (400 Preps)
$1,876.03
Applications

Total RNA isolation for:

  • RT-qPCR
  • RT-PCR
  • RNA sequencing
  • cDNA synthesis
  • Gene expression analysis

Benefits

  • Protocol & Components optimized to work with the iSWAB V2 Collection Kit.
  • High yield and quality total RNA purification
  • Adaptable to various automated liquid handling workstations
  • No toxic organic solvents

The HighPrep Total RNA Isolation Kit - iSWAB™ is a paramagnetic bead-based system optimized for efficient purification of high-quality total RNA from samples collected using the MAWI iSWAB V2 Collection Kit (ISWAB-RNA-v2) for numerous high-performance RNA downstream applications such as RT-PCR, microarrays, cDNA synthesis. As a paramagnetic bead-based system, the HighPrep Total RNA Isolation Kit - iSWAB™ can be used in a high-throughput format, hence adaptable to numerous commercially available workstations.

This is a short overview of the protocol, for all detailed protocols, please refer to PRODUCT DOCUMENTS
    1. Trasnfer 600uL of samples from the iSWAB-RNA-v2 Tubt to a DNase/RNase free Eppendorf tube.
    2. Centrifuge cells at 5000 x g for 10 mins and remove supernatant.
    3. Add 500uL LB Buffer to cells and incubate at RT Centrifuge sample at 10,000 x g for 10mins.
    4. Add CE Buffer to the lysate in a 1:1 ratio.

Add 10uL MAG-R4 Particles to each sample. Pipette mix and incubate at RT for 10mins.

  1. Wash steps using RW1 and RB2 Buffer. 
  2. DNA Digestion using DNAase I and DNAse I Digestion Buffer
  3. Elution using RNA Elution Buffer
Kit Components
  • LB Buffer
  • CE Buffer
  • RW1 Buffer
  • RB2 Buffer
  • DNase I
  • DNase I Digestion Buffer
  • RNA Elution Buffer
  • MAG-R4 Particles

Storage

MAG-R4 particles should be stored at 2-8°C. DNase A should be store at -20°C . All other components stored at 15-25°C.

Stability

14 months when kit components are stored as directed.

Disclaimers

For Research Use Only. Not for Use in Diagnostic Procedures.

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