MagBio’s New Long-Read Sequencing Technology: SFD-HT

SFD-HT (Short Fragment Depletor – High Throughput) is a patent-pending, tunable, paramagnetic bead-based reagent technology that optimizes input DNA for long-read sequencing workflows. By selectively depleting short DNA fragments, SFD-HT increases average read length and maximizes sequencing efficiency in complex genomic applications. Designed for both manual and automated use, it enables seamless implementation from benchtop research to high-throughput processing. SFD-HT is compatible with Oxford Nanopore Technologies (ONT), PacBio, and emerging long-read sequencing platforms, and is available for early evaluation and OEM integration discussions.

By depleting short DNA fragments, SFD-HT enriches samples for the high-integrity, long DNA fragments essential for a range of advanced genomic applications, including:

• Structural Variant Analysis: Enabling long contiguous reads that span large deletions, duplications, inversions, and complex rearrangements often missed with shorter fragments.
• De Novo Genome Assembly: Enriching for the long DNA fragments needed to resolve repetitive regions and support chromosome-scale contiguity in complex genomes.
• Cancer Genomics Research: Supporting detection of large-scale genomic alterations and fusion events by enriching for fragments that span structural breakpoints.
• Rare Disease Research: Preserving long-range genomic information needed to identify de novo structural variants and phase mutations in challenging, repeat-rich regions.
• Metagenomics and Microbiome Studies: Enriching for DNA fragments long enough to improve differentiation of closely related microbial strains and support assembly of high-quality metagenome-assembled genomes (MAGs).

By enabling tunable depletion of shorter DNA fragments, SFD-HT gives researchers greater control over input DNA optimization for specific long-read sequencing applications and library preparation workflows. With SFD-HT, MagBio continues to expand its portfolio of paramagnetic bead-based technologies designed to improve genomic workflow performance and advance next-generation sequencing innovation. Like all MagBio core technologies, SFD-HT is available through direct customer supply, distribution partnerships, licensing, OEM integration, and white-label opportunities.

Learn More About SFD-HT Early Adoption & Collaboration Inquiry