MagQuant™ Plus DNA Kit

magnetic beads based kit for normalization of DNA concentration, and quantitation of DNA for NGS and other applications


Grouped product items
Cat.# Product Name Price Qty
MQP-50096 MagQuant Plus™ DNA Kit (96 Preps)
$265.17
MQP-50384 MagQuant™ Plus DNA Kit (384 Preps)
$509.13
MQP-51920 MagQuant Plus™ DNA Kit (1920 Preps)
$1,697.09
Applications

DNA quantitation and normalization of DNA concentration for:

  • Next Gen Sequencing library construction
  • PCR, qPCR

Benefits

  • Rapid and reliable quantitation and normalization of DNA
  • No centrifugation step, no filtration step
  • Eliminates the need for standard curve
  • Allowing the processing of pool DNA samples from various sources

The MagQuant™ Plus DNA Kit utilizes paramagnetic bead-based technology for genomic DNA quantitation and concentration normalization.  The DNA normalization occurs during the purification process.  Standard methods of DNA quantitation require the use of a standard curve derived from DNA samples of known concentrations; processing DNA samples from various sources would require extensive work and reagent use. MagQuant™ Plus DNA ‘s proprietary technology allows to obtain a given DNA quantitation and required concentration of DNA samples regardless of the source by adding a specific number of beads to the DNA sample.

This is a short overview of the protocol, for all detailed protocols, please refer to PRODUCT DOCUMENTS
  1. Transfer DNA to be normalized to a 96-well PCR plate
  2. For Library Normalization: Add NB Buffer and Mag-L1 Particles. For gDNA Normalization: Add SWB Buffer and Mag-L1 Particles
  3. Bind appropriate amount of DNA to beads
  4. For Library Normalization: Add NB Buffer. For gDNA Normalization: Add SWB Buffer
  5. Wash using MB Wash Buffer 2 times
  6. Elution done using MB Elution Buffer
  7. Store the DNA at -20°C.
Kit Components

  • MAG-L1 Particles
  • SWB Buffer
  • NB Buffer
  • MB Elution Buffer
  • MB Wash Buffer
Storage

MAG-L1 particles should be stored at 2-8°C.  All other components stored at 15-25°C.

Stability

Stable for 14 months when individual reagents stored at appropriate temperatures.

Disclaimers

For Research Use Only. Not for Use in Diagnostic Procedures.

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