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SFD-10HT
Eliminates DNA Fragments <10 kb
The First Magnetic bead-based reagent for efficient elimination of short DNA Fragments
Short Fragment Depletor-10 High Throughput (SFD-10HT) reagent is a bead-based reagent used for size selection and purification of high molecular weight DNA in long read sequencing. It can significantly improve the mean read length by near elimination of DNA fragments below 10 kb. This kit is suitable for amplicons, sheared/fragmented DNA and genomic DNA. SFD-10HT working concentration range is 10-150 ng/µL.
Performance Data | Comparative Data | Request a Quote
- High Molecular Weight DNA: purification and concentration
- Sheared/fragmented DNA: elimination of fragments <10 kb
- Library cleanup and rapid size selection >10 kb
- Enhancement of DNA mean read length by elimination of short fragments (<10 kb) prior to library preparation
- During SFD-10HT size selection and short fragment elimination, DNA is purified, which helps eleminate contaminants that may be carried over from the extraction process.
- Concentrates dilute HMW DNA samples
- Tolerant to a wide range of DNA input range (10-150 ng/µL)
- Applicable in a wide variety of sample types: HMW DNA, fragmented DNA, sheared DNA, and PCR amplicons/libraries
- Bead-based - no centrifugation
- Efficient scale-up and flexibility - amenable to automation
- Reproducible results
- High recovery efficiency (50-90%) depending on quality and concentration of input DNA
- Saves time - protocol less than 65 minutes
The Short Fragment Depletor-10 High Throughput (SFD-10HT) reagent is designed for the cleanup and size selection of sheared or fragmented DNA in third-generation sequencing. SFD-10HT uses bead-based size selection to drastically eliminate short DNA fragments below 10 kb. Short DNA fragments (<10 kb) are progressively eliminated and very large fragments are enriched to improve the mean read length. SFD-10HT is an ideal solution for enhancement of reads' quality in long-read sequencing workflows.
This is a short overview of the protocol. For a detailed protocol, please refer to PRODUCT DOCUMENTS.
1. Add SFD-10HT reagent to the reaction mixture
2. Bind DNA to paramagnetic beads
3. Separation of beads from the supernatant
4. Wash beads with Ethanol to remove contaminants
5. Elute purified PCR products or other DNA fragments from beads
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Main functions |
Progressively eliminates DNA fragments <10 kb and rapidly selects long DNA libraries |
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Starting material (Sample matrix) |
Sheared/fragmented DNA, amplicons/libraries, HMW DNA |
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Starting amount |
10-150 ng/µL of DNA |
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DNA recovery |
50-90% depending on input DNA quality and concentration |
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Process method |
Manual or automation |
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Instrument |
Adaptable to most nucleic acid purification instruments but a ready-made script for Kingfisher Flex is available upon request |
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Purification method |
Magnetic bead-based technology |
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DNA purity |
DNA eluted is ready for downstream processes such as library preparation for third generation sequencing |
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Elution volume |
10 ng/µL or above |