SFD-HT (Short Fragment Depletor – High Throughput)

Tunable Reagent for Efficient Elimination of DNA Fragments <10 kb, <25 kb and <40 kb

Short Fragment Depletor – High Throughput (SFD-HT) is a new, patent-pending magnetic bead–based reagent used for size selection and purification of HMW DNA for long-read sequencing. It is designed for tunable depletion of short DNA fragments, enabling depletion of fragments below 10 kb, 25 kb, or 40 kb. SFD-HT is being developed as a high throughput & automation friendly alternative to Company PB’s SRE-XS, SRE, and SRE-XL reagents for PacBio® and Oxford Nanopore® long-read sequencing workflows and is intended to give long-read sequencing labs in research, clinical, industrial, and public health greater control over size selection of large DNA fragments using a tunable reagent and an automation-ready protocol.

Performance Data   SFD-HT Early Adopter & Collaboration Program Form 

Why should you Test SFD-HT?

• Efficient short-fragment depletion with configurable size thresholds
  Eliminates short DNA fragments to enrich long DNA fragments, with 10 kb, 25 kb, or 40 kb size-selection options.
• Preservation of ultra-long DNA fragments
  Enriches long fragments while maintaining representation of ultra-long fragments (e.g. >100 kb)
• Broad sample compatibility
  Applicable to HMW genomic DNA, sheared or fragmented DNA, and libraries.
• High recovery efficiency
  Delivers 50–90% recovery, depending on the quality and concentration of the input DNA.
• Rapid and flexible protocol
  Total processing time of ~65 minutes, with a flexible input range of 20–150 ng/µL.
• Performance
  Improves mean read length, median read length, and N50 values.
• Workflow
  Bead-based, centrifugation-free protocol compatible with magnetic racks and liquid-handling systems, enabling quick, consistent, automatable size selection for high-throughput labs.
• Versatility
  Handles diverse sample types and input ranges with tunable cut-offs (10 kb, 25 kb, 40 kb) to fit different library prep workflows and sequencing platforms.

Interested in testing SFD-HT?

Then join the SFD-HT Early Adopter & Collaboration Program

MagBio Genomics invites qualified laboratories and biotech companies to participate in our SFD-HT Early Adopter & Collaboration Program. As an early partner, you will test SFD-HT during its innovation, development and testing phase, providing feedback that directly shapes the product and its applications in long-read sequencing.

How your Lab will Benefit from the Collaboration Program.

• Early Access to SFD-HT
  Integrate this new short-fragment depletion technology into your long-read workflows ahead of the broader market, gaining a competitive edge.
• Direct Collaboration with MagBio R&D
  Work directly with our scientists to shape the product's development, optimize performance on your samples, and influence future product features.
• Discounted Pricing at Launch
  Secure a special discounted rate when SFD-HT is fully launched, in recognition of your contribution as an early collaborator, ensuring favorable long-term cost-effectiveness.
• Enhanced Visibility
  Be recognized as a contributor to next-generation long-read sequencing innovation through collaborative opportunities.

SFD-HT Early Adopter & Collaboration Program Form