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HighPrep PCR

Magnetic bead-based reagent for manual and automated post-PCR and post-enzymatic reaction cleanup, NGS library cleanup, and reliable DNA size selection.

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HighPrep Viral DNA & RNA Kit

Magnetic bead based kit optimized for extraction of RNA from a variety of viral samples - Swabs, whole blood, serum, plasma, saliva & other body fluids.

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MagQuant Plus DNA Kit V2

Magnetic beads based kit for normalization of DNA concentration, and quantitation of DNA for NGS and other applications

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HighPrep Total RNA Plus Kit

Magnetic bead-based kit designed to extract high-quality total RNA from mammalian tissues and cultured cells.

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HighPrep DTR

Magnetic beads based reagent for manual and automated Sanger sequencing reaction clean-up for both ABI and MegaBACE sequencing platforms.

Grouped product items
Cat.# Product Name Price Qty
DT-70005 HighPrep DTR (5 mL)
DT-70050 HighPrep DTR (50 mL)
DT-70250 HighPrep DTR (250 mL)
DT-70500 HighPrep DTR (500 mL)
DT-70005E HighPrep DTR - DX (5 mL)
DT-70050E HighPrep DTR - DX (50 mL)
DT-70250E HighPrep DTR - DX (250 mL)
DT-70500E HighPrep DTR - DX (500 mL)

Sanger sequencing reaction clean-up / dye terminator removal for:

  • Sanger sequencing on ABI platforms
  • Sanger sequencing on MegaBACE platforms


  • Rapid and reliable clean-up of sequencing reactions
  • High DNA recovery yields, long sequence readings
  • Flexible - can be used in any format
  • No centrifugation step, no filtration step
  • Adaptable to common liquid handling workstations

The HighPrep DTR  is a high performance paramagnetic bead-based reagent for BigDye™ terminator removal from Sanger sequencing reactions for reliable and long readings of DNA sequences. The HighPrep DTR  consists of a selective binding of DNA to the paramagnetic beads, followed with washing off nucleotides, primers, and non-targeted amplicons, and finally elution of pure DNA. HighPrep DTR  is an excellent choice for both manual and fully automated purification of sequencing reaction products. The protocol can be adapted to common liquid handling workstation.

BigDye is a registered trademark of Applied Biosystems

This is a short overview of the protocol, for all detailed protocols, please refer to PRODUCT DOCUMENTS
  1. Add HighPrep™DTR reagent to sample
  2. Add freshly prepared 85% ethanol
  3. Bind sample to paramagnetic beads
  4. Wash beads with 85% ethanol to remove contaminants
  5. Elute purified DNA from beads
Features Specifications
Main functions Removes unincorporated dye terminators from Sanger sequencing reactions
Starting material (Sample matrix) DNA, PCR products, cDNA, gDNA
Starting amount Scalable
Process method Manual or automation
Purification method Magnetic bead-based technology
Application Sanger sequencing on ABI platforms and MegaBACE platforms
Elution Volume 15 uL and above

  1. An economical and efficient method of post reaction cleanup of labeled dye terminator sequencing products. Rajesh R Kundapur, Vijay Nema
  2. Knock-In of a 25-Kilobase Pair BAC-Derived Donor Molecule 9 by Traditional and CRISPR/Cas9-Stimulated Homologous Recombination. Tiffany Leidy-Davis, Kai Cheng, Leslie O. Goodwin, Judith L. Morgan, Wen Chun Juan, Xavier Roca, Sin-Tiong Ong, David E. Bergstrom
  3. Nuclear introgression without mitochondrial introgression in two turtle species exhibiting sex-specific trophic differentiation. Sarah M. Mitchell,nLaura K. Muehlbauer, Steven Freedberg
  4. A European epidemiological survey of Vibrio splendidus clade shows unexplored diversity and massive exchange of virulence factors. Nasfi H, Travers MA, de Lorgeril J, Habib C, Sannie T, Sorieul L, Gerard J, Avarre JC, Haffner P, Tourbiez D, Renault T, Furones D, Roque A, Pruzzo C, Cheslett D, Gdoura R, Vallaeys T.
  5. Factors affecting Sanger sequencing robustness and data quality. Patrick Warner, Shea Anderson, Archana Deshpande, Daryl M. Gohl, Kenneth B. Beckman

Want to share one more referenece? Write us an email.