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HighPrep PCR
Direct and Cost-effective Alternative to the Leading SPRI Bead-Based Reagent
Paramagnetic bead-based post PCR clean-up reagent designed for efficient DNA purification, tight DNA size selection, NGS library clean-up, and Oxford Nanopore Technologies® (ONT) Library Preparation Cleanup. With no centrifugation or filtration required, it enables a simple, scalable workflow for both manual and fully automated sample processing.
NYU GenCore Comparison: HighPrep PCR Beads as an AMPureXP Alternative
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Post-PCR and post-enzymatic reaction clean-up used for/during:
- NGS library preparation
- DNA size selection for NGS
- Microarrays
- PCR
- Restriction enzyme digestions, adapter ligations
- Cloning
- Rapid and reliable post-PCR and DNA clean-up reagent
- Achieve uniform and consistent DNA fragments
- High recovery of amplicons >100 bp
- Uniform fragments size distribution
- Adaptable to high throughput liquid handling workstations
- What is HighPrep PCR used for?
A paramagnetic bead-based reagent for post-PCR clean-up, NGS library clean-up, Oxford Nanopore Technologies® (ONT) library preparation and DNA size selection. The purified DNA is ready for downstream applications, such as sequencing, qPCR, cloning, microarrays and more. - Does it need a centrifuge or spin columns?
No. It uses a magnetic bead-based workflow with an ethanol wash; no centrifugation or filtration is required. So, it’s faster, uses fewer consumables, and adapts easily to automation. - What DNA recovery and fragment size can I expect?
Expect >70% DNA recovery. At a 1.8x bead ratio, the reagent provides reliable recovery of DNA fragments ≥100 bp. Adjusting the bead-to-sample ratio shifts the cut-off for precise DNA size selection. - Can I use it for NGS library prep and size selection?
Yes. It’s designed for NGS library cleanup and tunable DNA size selection, giving uniform, consistent fragment distribution for sequencing workflows. - Is it compatible with automated liquid handlers?
Yes. It’s adaptable to most nucleic-acid purification instruments, and a ready-made KingFisher™ Flex script is available on request; reducing hands-on time and pipetting error. - What sample types and inputs does it work with?
It works with DNA, PCR products, cDNA and genomic DNA (gDNA), with scalable starting amounts. It removes salts, primers, primer-dimers and unincorporated dNTPs. - What’s the difference between HighPrep PCR and HighPrep PCR-DX product categories?
The HighPrep PCR - DX product category is the regulatory-registered CE-IVD grade for diagnostic workflows and the HighPrep PCR product category is for Research Use Only (RUO). - Is it suitable for clinical and diagnostic labs?
The HighPrep PCR - DX product category is CE-IVD registered and is suitable for clinical and diagnostic labs. - Can I try it before buying?
Yes, you may request a free sample to validate it in your workflow first. - How should it be stored, and what is the shelf life?
It’s stable for 18 months when stored at 2-8°C. DO NOT FREEZE.
HighPrep PCR is a paramagnetic beads-based post PCR clean-up reagent designed for efficient purification of amplicons and tight size-specific selection of DNA fragments. The purification consists of removal of salts, primers, primer-dimers, dNTPs, as DNA fragments are selectively bound to the magnetic bead particles. Highly purified DNA is eluted with low salt elution buffer or water which can be used directly for downstream applications, such as library construction, next generation sequencing (NGS), Sanger sequencing, cloning, restriction digestions, adapter ligation, microarrays, and so on. This protocol can be used for manual procedures as well as in automation.
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This is a short overview of the protocol, for all detailed protocols, please refer to PRODUCT DOCUMENTS
Add reagent
Add HighPrep PCR to the reaction mixture
Bind
Bind DNA to paramagnetic beads
Separate
Separate beads and DNA from contaminants
Wash
Wash beads with 80% ethanol to remove contaminants
Elute
Elute purified PCR products or other DNA fragments from beads
Add reagent
Add HighPrep PCR to the reaction mixture
Bind
Bind DNA to paramagnetic beads
Separate
Separate beads and DNA from contaminants
Wash
Wash beads with 80% ethanol to remove contaminants
Elute
Elute purified PCR products or other DNA fragments from beads
| Features | Specifications |
|---|---|
| Main function |
Purifies DNA and amplicons for next generation sequencing (NGS) and other applications. Selectively binds fragments ≥100 bp depending on sample to bead ratio, and performs size selection by changing the bead ratio. |
|
Starting material |
DNA, PCR products, cDNA, gDNA |
| Starting amount | Scalable |
| DNA recovery | >70% |
| Process method | Manual or automated |
| Instrument |
Adaptable to most nucleic acid purification instruments, but a ready-made script for KingFisher Flex is available upon request. |
| Downstream-ready |
Real time quantitative PCR (qPCR)/PCR, next generation sequencing (NGS), Sanger sequencing, cloning, restriction digestions, microarrays, etc. |
| Elution volume | 15 µl or above |
| Purification method | Magnetic bead-based technology |
| Storage temperature | Store at 2-8°C. DO NOT FREEZE |
| Shelf life | Stable for 18 months when stored at 2-8°C. |
Trusted in Published Genomics Research
HighPrep PCR is trusted by private and academic institutions worldwide and has been cited in over 6,000 publications. Selected references are listed below.
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