HighPrep™ PCR Clean-up System

Magnetic beads based reagent for manual and automated post-PCR and post-enzymatic reaction clean-up, for NGS library clean-up and reliable amplicon size selection.


Grouped product items
Cat.# Product Name Price Qty
AC-60005 HighPrep™ PCR (5 mL)
$101.83
AC-60050 HighPrep™ PCR (50 mL)
$526.10
AC-60250 HighPrep™ PCR (250 mL)
$2,156.36
AC-60500 HighPrep™ PCR (500 mL)
$3,503.42
Applications

Post-PCR and post-enzymatic reaction clean-up used for/during:

  • NGS library preparation
  • Amplicon size selection for NGS
  • Microarrays
  • PCR
  • Sanger sequencing
  • Restriction enzyme digestions, adapter ligations
  • Cloning

Benefits

  • Rapid and reliable post-PCR and post enzymatic reaction clean-up
  • Efficient wash from contaminants
  • High recovery of amplicons >100bp
  • Uniform fragments size distribution
  • Adaptable to high throughput liquid handling workstations

The HighPrep™ PCR Clean-up System is a paramagnetic beads-based post PCR clean-up reagent designed for an efficient purification of DNA fragments and tight size-specific selection of amplicons. The purification consists of removal of salts, primers, primer-dimers, dNTPs, as DNA fragments are selectively bound to the magnetic bead particles. Highly purified DNA is eluted with low salt elution buffer or water which can be used directly for downstream applications, such as library construction, next generation sequencing (NGS), Sanger  sequencing, cloning, restriction digestions, adapoter ligation, microarrays, and so on. This protocol can be used for manual procedure as well as as a guideline for adapting the kit to automatic liquid handling instruments. The HighPrep™ PCR Clean-up Reagent is perfectly suited for amplicon size selection, and NGS library preparation for sequencing as well as for post PCR clean-up of shorter amplicons or restriction enzyme digestion products.

  • Efficient recovery of amplicons achieved with HighPrep™ PCR Clean-up System

Efficient recovery of amplicons achieved with HighPrep™ PCR Clean-up System

This is a short overview of the protocol, for all detailed protocols, please refer to PRODUCT DOCUMENTS
  1. Add HighPrep™ PCR reagent to the reaction mixture
  2. Bind DNA to paramagnetic beads
  3. Separation of beads + DNR from contaminants
  4. Wash beads with Ethanol to remove contaminants
  5. Elute purified PCR products or other DNA fragments from beads
Kit Components

  • HighPrep™ PCR Clean-up solution
Storage

Store at 4°C to 8°C | DO NOT FREEZE

Stability

14 months when stored as directed.

Disclaimers

For Research Use Only. Not for Use in Diagnostic Procedures.

Number of reactions
In 96 Well Format
PCR Reaction Volume Number of reactions
10 µL 2,778
20 µL 1,389
50 µL 556
In 384 Well Format
PCR Reaction Volume Number of reactions
5 µL 5,556
7 µL 3,968
10 µL 2,778
14 µL 2,000

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  2. RNA-seq Analysis of Irrigated vs. Water Stressed Transcriptomes of Zea mays Cultivar Z59. Divya Bhanu, Kandasamy Ulaganathan, Arun K. Shanker and S. Desai
  3. Nucleotide pools dictate the identity and frequency of ribonucleotide incorporation in mitochondrial DNA. Anna-Karin Bergl, Clara Navarrete, Martin K. M. Engqvist, Emily Hoberg, Zsolt Szilagyi, Robert W. Taylor, Claes, M. Gustafsson, Maria Falkenberg, Anders R. Clausen 
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  7. Taxonomic description and genome sequence of Bacillus campisalis sp. nov., a member of the genus Bacillus isolated from a solar saltern. Rajendran Mathan Kumar,Gurwinder Kaur, Anand Kumar, Monu Bala, Nitin Kumar Singh, Navjot Kaur, Narender Kumar and Shanmugam Mayilraj
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  10. Having older siblings is associated with gut microbiota development during early childhood. Martin Frederik Laursen, Gitte Zachariassen, Martin Iain Bahl, Anders Bergström, Arne Høst, Kim F. Michaelsen and Tine Rask Licht
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  14. Phosphopantothenoylcysteine Synthetase from Escherichia coli. Erick Strauss, Cynthia Kinsland, Ying Ge, Fred W. McLafferty, and Tadhg P. Begley
  15. Microbial diversity and community respiration in freshwater sediments influenced by artificial light at night. Franz Hölker, Christian Wurzbacher, Carsten Weißenborn, Michael T. Monaghan, Stephanie I. J. Holzhauer, Katrin Premke
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  20. Taxonomic description and genome sequence of Salinicoccus sediminis sp. nov., a halotolerant bacterium isolated from marine sediment. Rajendran Mathan Kumar, Gurwinder Kaur, Narender Kumar, Anand Kumar, Nitin Kumar Singh, Monu Bala, Navjot Kaur, Shanmugam Mayilraj 
  21. The role of the intestinal microbiota in pneumonia and sepsis. Lankelma, J.M.
  22. Taxonomic description and genome sequence of Salinicoccus sediminis sp. nov., a halotolerant bacterium isolated from marine sediment. Rajendran Mathan Kumar, Gurwinder Kaur, Narender Kumar, Anand Kumar, Nitin Kumar Singh, Monu Bala, Navjot Kaur and Shanmugam Mayilraj.
  23. Tracking replication enzymology in vivo by genome-wide mapping of ribonucleotide incorporation. Anders R Clausen, Scott A Lujan, Adam B Burkholder, Clinton D Orebaugh, Jessica S Williams, Maryam F Clausen, Ewa P Malc, Piotr A Mieczkowski, David C Fargo, Duncan J Smith & Thomas A Kunkel
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