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MagQuant Plus DNA Kit V2
Magnetic bead based kit for normalization of DNA concentration, and quantitation of DNA for NGS and other applications
DNA quantitation and normalization of DNA concentration for:
- Next Gen Sequencing library construction
- Amplicon normalization
- Genomic DNA normalization
- PCR, qPCR
- Rapid and reliable quantitation and normalization of DNA
- No centrifugation step, no filtration step
- Eliminates the need for standard curve
- Allowing the processing of pool DNA samples from various sources
- Equalizing concentration of genomic DNA input for DNA libray construction to help produce consistent and reliable NGS data without tedious initial input DNA quantitation.
The MagQuant Plus DNA Kit V2 utilizes paramagnetic bead-based technology for genomic DNA quantitation and concentration normalization. The DNA normalization occurs during the purification process. Standard methods of DNA quantitation require the use of a standard curve derived from DNA samples of known concentrations; processing DNA samples from various sources would require extensive work and reagent use. MagQuant Plus DNA V2‘s proprietary technology allows to obtain a given DNA quantitation and required concentration of DNA samples regardless of the source by adding a specific number of beads to the DNA sample.
This is a short overview of the protocol, for all detailed protocols, please refer to PRODUCT DOCUMENTS
- Transfer DNA to be normalized to a 96-well PCR plate
- Add Binding Buffer & MAG-C7 Beads
- Bind appropriate amount of DNA to beads
- Wash using Ethanol 2 times
- Elution done using MB Elution Buffer
- Store the DNA at -20°C.
| Starting material (Sample matrix) | DNA fragments, PCR products, and gDNA |
| DNA input | Minimum DNA input for DNA fragments or genomic DNA normalization is 800 ng and for amplicon normalization is 300 ng. |
| DNA Purity Requirements | A260/A230 should be around 1.7 or above |
| Process method | Manual |
| Purification method | Magnetic bead-based technology |
| Application | Next-Generation Sequencing (NGS) |
| Elution Volume | 25-50μl (adjust as needed) |
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