VIRAL RNA EXTRACTION FOR COVID-19

Featured Products

HighPrep PCR

Magnetic bead-based reagent for manual and automated post-PCR and post-enzymatic reaction cleanup, NGS library cleanup, and reliable DNA size selection.

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HighPrep Viral DNA & RNA Kit

Magnetic bead based kit optimized for extraction of RNA from a variety of viral samples - Swabs, whole blood, serum, plasma, saliva & other body fluids.

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MagQuant Plus DNA Kit V2

Magnetic beads based kit for normalization of DNA concentration, and quantitation of DNA for NGS and other applications

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HighPrep Total RNA Plus Kit

Magnetic bead-based kit designed to extract high-quality total RNA from mammalian tissues and cultured cells.

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MagBio offers free samples of all kits & reagents for testing so that you can validate our products and ensure they suit your workflow before committing to any purchases.

Click on the link below or give us a call at (301) 302-0144 to request your free sample today!

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Latest News

. July 2, 2024

Comparison of HighPrep PCR PB and AMPure PB Performance in PacBio’s WGS HiFi Sequencing Technology

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. May 20, 2024

MagBio Launches the new Long-read sequencing extration kit

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. June 4, 2024

MagBio's new Long-read sequencing cleanup kit

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MagQuant Plus DNA Kit V2

Magnetic bead based kit for normalization of DNA concentration, and quantitation of DNA for NGS and other applications


Grouped product items
Cat.# Product Name Price Qty
MQP-50096 MagQuant Plus DNA Kit V2 (96 Preps)
$425.70
MQP-50384 MagQuant Plus DNA V2 (384 Preps)
$1,276.04
MQP-51920 MagQuant Plus DNA Kit V2 (1920 Preps)
$5,104.15
MQP-55000 MagQuant Plus DNA Kit V2 (5000 Preps)
$12,250.00
Applications

DNA quantitation and normalization of DNA concentration for:

  • Next Gen Sequencing library construction
  • Amplicon normalization
  • Genomic DNA normalization
  • PCR, qPCR

Benefits

  • Rapid and reliable quantitation and normalization of DNA
  • No centrifugation step, no filtration step
  • Eliminates the need for standard curve
  • Allowing the processing of pool DNA samples from various sources
  • Equilizing input genomic DNA concentration for DNA libray construction to help produce consistent and reliable NGS data without tedious initial input DNA quantitation.

The MagQuant Plus DNA Kit V2 utilizes paramagnetic bead-based technology for genomic DNA quantitation and concentration normalization.  The DNA normalization occurs during the purification process.  Standard methods of DNA quantitation require the use of a standard curve derived from DNA samples of known concentrations; processing DNA samples from various sources would require extensive work and reagent use. MagQuant™ Plus DNA V2‘s proprietary technology allows to obtain a given DNA quantitation and required concentration of DNA samples regardless of the source by adding a specific number of beads to the DNA sample.

This is a short overview of the protocol, for all detailed protocols, please refer to PRODUCT DOCUMENTS
  1. Transfer DNA to be normalized to a 96-well PCR plate
  2. Add Binding Buffer & MAG-C7 Beads
  3. Bind appropriate amount of DNA to beads
  4. Wash using Ethanol 2 times
  5. Elution done using MB Elution Buffer
  6. Store the DNA at -20°C.
Starting material (Sample matrix) DNA fragments, PCR products, and gDNA
DNA input Minimum DNA input for DNA fragments or genomic DNA normalization is 800 ng and for amplicon normalization is 300 ng.
DNA Purity Requirements A260/A230 should be around 1.7 or above
Process method Manual
Purification method Magnetic bead-based technology
Application Next-Generation Sequencing (NGS)
Elution Volume 25-50μl (adjust as needed)

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