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MagQuant Plus DNA V2
Magnetic bead based kit for normalization of DNA concentration, and quantitation of DNA for NGS and other applications
DNA quantitation and normalization of DNA concentration for:
- Next Gen Sequencing library construction
- Amplicon normalization
- Genomic DNA normalization
- PCR, qPCR
- Rapid and reliable quantitation and normalization of DNA
- No centrifugation step, no filtration step
- Eliminates the need for standard curve
- Allowing the processing of pool DNA samples from various sources
- Equilizing input genomic DNA concentration for DNA libray construction to help produce consistent and reliable NGS data without tedious initial input DNA quantitation.
The MagQuant™ Plus DNA V2 Kit utilizes paramagnetic bead-based technology for genomic DNA quantitation and concentration normalization. The DNA normalization occurs during the purification process. Standard methods of DNA quantitation require the use of a standard curve derived from DNA samples of known concentrations; processing DNA samples from various sources would require extensive work and reagent use. MagQuant™ Plus DNA V2‘s proprietary technology allows to obtain a given DNA quantitation and required concentration of DNA samples regardless of the source by adding a specific number of beads to the DNA sample.
This is a short overview of the protocol, for all detailed protocols, please refer to PRODUCT DOCUMENTS
- Transfer DNA to be normalized to a 96-well PCR plate
- Add Binding Buffer & MAG-C7 Beads
- Bind appropriate amount of DNA to beads
- Wash using Ethanol 2 times
- Elution done using MB Elution Buffer
- Store the DNA at -20°C.
| Starting material (Sample matrix) | DNA fragments, PCR products, and gDNA |
| DNA input | Minimum DNA input for DNA fragments or genomic DNA normalization is 800 ng and for amplicon normalization is 300 ng. |
| DNA Purity Requirements | A260/A230 should be around 1.7 or above |
| Process method | Manual |
| Purification method | Magnetic bead-based technology |
| Application | Next-Generation Sequencing (NGS) |
| Elution Volume | 25-50μl (adjust as needed) |
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