HighPrep™ Blood & Tissue DNA Kit

Blood and tissue DNA kit, magnetic bead-based kit for high quality genomic DNA extraction from 50-250 µL of fresh or frozen whole blood, buffy coat, lysate of tissues, mouse tails, cultured cells, saliva, buccal swabs.


Grouped product items
Cat.# Product Name Price Qty
HPBTS-D96 HighPrep™ Blood & Tissue DNA Kit (96 Preps)
$222.74
HPBTS-D96x4 HighPrep™ Blood & Tissue DNA Kit (384 Preps)
$772.17
Applications

Blood and tissue gDNA extraction for:

  • PCR, Real-time PCR
  • Southern Blotting
  • Cloning, Genotyping
  • Sequencing

Benefits

  • High-quality DNA from blood and tissue samples
  • No organic extraction or alcohol precipitation
  • Complete removal of contaminants and inhibitors
  • Adaptable to various automated workstations

Blood and Tissue DNA Kit has been designed to isolate high quality genomic DNA from 50-250 µL of fresh or frozen whole blood, buffy coat, lysate of tissues, mouse tails, cultured cells, saliva, buccal swabs.

HighPrep™ Blood & Tissue DNA Kit utilizes paramagnetic beads to isolate genomic DNA (gDNA) from various sample types including tissue, whole blood, saliva and cells; resulting in high quantity and quality DNA usable in various genomic downstream applications. HighPrep™ Blood & Tissue DNA Kit could be used for few samples as well as in high throughput setting on automated workflow.

  • High gDNA yield and pure samples extracted with HighPrep™ Blood & Tissue DNA Kit

Performance of HighPrep Blood & Tissue DNA Kit - high yield and pure samples

  • Higher yields of gDNA from tissue samples achieved with HighPrep™ Blood & Tissue DNA Kit

10 mg of fresh chicken liver was used for this experiment. 200 μl Elution Buffer for both kits. 5 μl DNA Solution used in each gel well.

DNA Extraction from Tissue

  • Higher yields of high quality gDNA from blood samples achieved with HighPrep™ Blood & Tissue DNA Kit

150 μl of whole blood was used for this experiment. For Competitor kit: 200 μl Elution Buffer used. 10 μl DNA solution used for each well.
For MagBio kit 100 μl Elution Buffer used. 5 μl DNA solution used for each well.

DNA Extraction from Blood

This is a short overview of the protocol, for detailed protocols, please refer to PRODUCT DOCUMENTS
  1. Add blood or tissue sample to well of 1.2 mL deep-well plate.  Bring sample volume up to with PBS or with MB Elution Buffer.
  2. Add Pro K Solution
  3. Add Lysis Buffer
  4. Add 100 % ethanol and MAG-S1 particles
  5. Wash by adding 70% ethanol to the sample
  6. Elution done by using MB Elution Buffer
  7. Store DNA at -20°C.
Kit Components
  • AS Buffer
  • TS Buffer
  • HSW Buffer
  • MB Elution Buffer
  • Pro K Solution
  • MAG-S1 particles
Storage

MAG-S1 particles and Pro K solution should be stored at 2-8°C. All other components stores at 15-25°C.

Stability

14 months when kit components are stored as directed.

Disclaimers

For Research Use Only. Not for Use in Diagnostic Procedures.

No references yet. Want to share one? Write us an email.