VIRAL RNA EXTRACTION FOR COVID-19

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HighPrep PCR

Magnetic bead-based reagent for manual and automated post-PCR and post-enzymatic reaction cleanup, NGS library cleanup, and reliable DNA size selection.

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HighPrep Viral DNA & RNA Kit

Magnetic bead based kit optimized for extraction of RNA from a variety of viral samples - Swabs, whole blood, serum, plasma, saliva & other body fluids.

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SFD-10HT

Magnetic bead-based reagent used for elimination of DNA fragments <10 kb. It is used for size selection & purification of high molecular weight DNA in long-read sequencing

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HighPrep RNA Elite

Magnetic beads based reagent for manual and automated clean-up or concentration of RNA and single-stranded cDNA after enzymatic reactions or prior sequencing.

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MagBio offers free samples of all kits & reagents for testing so that you can validate our products and ensure they suit your workflow before committing to any purchases.

Click on the link below or give us a call at (301) 302-0144 to request your free sample today!

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Latest News

SFD-10HT Launch depletion of DNA fragments below 10 kb

. Oct 10, 2024

MagBio Launches SFD-10HT (Short Fragment Depletor - 10 High Throughput) l Bead-based Reagent That Progressively Depletes DNA Fragments <10 kb

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. May 20, 2024

MagBio Launches the new Long-read sequencing extration kit

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. June 4, 2024

MagBio's new Long-read sequencing cleanup kit

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HighPrep Blood & Tissue DNA Kit

Blood and tissue DNA kit, a magnetic bead-based kit for high-quality genomic DNA extraction from 50-250 µL of fresh or frozen whole blood, buffy coat, lysate of tissues, mouse tails, cultured cells, saliva, buccal swabs.


Grouped product items
Cat.# Product Name Price Qty
HPBTS-D96 HighPrep Blood & Tissue DNA Kit (96 Preps)
$291.05
HPBTS-D96x4 HighPrep Blood & Tissue DNA Kit (384 Preps)
$1,008.98
Applications

Blood and tissue gDNA extraction for:

  • PCR, Real-time PCR
  • Southern Blotting
  • Cloning, Genotyping
  • Sequencing

Benefits

  • High-quality DNA from blood and tissue samples
  • No organic extraction or alcohol precipitation
  • Complete removal of contaminants and inhibitors
  • Adaptable to various automated workstations

Blood and Tissue DNA Kit has been designed to isolate high quality genomic DNA from 50-250 µL of fresh or frozen whole blood, buffy coat, lysate of tissues, mouse tails, cultured cells, saliva, buccal swabs.

HighPrep Blood & Tissue DNA Kit utilizes paramagnetic beads to isolate genomic DNA (gDNA) from various sample types including tissue, whole blood, saliva and cells; resulting in high quantity and quality DNA usable in various genomic downstream applications. HighPrep Blood & Tissue DNA Kit could be used for few samples as well as in high throughput setting on automated workflow.

  • High gDNA yield and pure samples extracted with HighPrep Blood & Tissue DNA Kit

Performance of HighPrep Blood & Tissue DNA Kit - high yield and pure samples

  • Higher yields of gDNA from tissue samples achieved with HighPrep Blood & Tissue DNA Kit

10 mg of fresh chicken liver was used for this experiment. 200 μl Elution Buffer for both kits. 5 μl DNA Solution used in each gel well.

DNA Extraction from Tissue

  • Higher yields of high quality gDNA from blood samples achieved with HighPrep Blood & Tissue DNA Kit

150 μl of whole blood was used for this experiment. For Competitor kit: 200 μl Elution Buffer used. 10 μl DNA solution used for each well.
For MagBio kit 100 μl Elution Buffer used. 5 μl DNA solution used for each well.

DNA Extraction from Blood

This is a short overview of the protocol, for detailed protocols, please refer to PRODUCT DOCUMENTS
  1. Add blood or tissue sample to well of 1.2 mL deep-well plate.  Bring sample volume up to with PBS or with MB Elution Buffer.
  2. Add Pro K Solution
  3. Add Lysis Buffer
  4. Add 100 % ethanol and MAG-S1 particles
  5. Wash by adding 70% ethanol to the sample
  6. Elution done by using MB Elution Buffer
  7. Store DNA at -20°C.
Purification method Magnetic bead-based technology
Elution Volume ≥ 50 µl
Size Range Capable of recovering genomic DNA sized fragments > 60 kb. If present, parasitic, microbial, and viral DNA will also be recovered.
Purity High quality and pure DNA- ready for all sensitive downstream applications such as Real time quantitative PCR, Reverse Transcriptase PCR, PCR, Next Generation Sequencing, genotyping,
A260/A280 and A260/A230: >1.8.
Yield Mammalian tissues yields up to 25 ug of DNA in 50 ul of elution volume: Human whole blood will yield 3-7 µg DNA per 100 ul of blood sample.

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