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HighPrep Viral RNA Kit – KingFisher™ Format
Optimized For COVID-19 | KingFisher™ MVP II Alternative
The HighPrep™ Viral RNA Isolation Kit is a magnetic bead-based kit specifically designed to work with KingFisher™ platforms. Use this kit to separate viral nucleic acids from serum, plasma, swabs, VTM, saliva and other body fluids.
Like all MagBio products, this isolation kit purifies rapidly and reliably, so you can focus on your work. It’s optimized to isolate the SARS-COV2 RNA, it’s an essential item for ongoing COVID research.
Sensitivity Data | Comparative Data | VTM COMPATIBILITY
Viral RNA isolation for:
- RT-qPCR; RT-PCR, PCR
- One-Step RT-qPCR
- Virus detection, genotyping
- Viral load monitoring,
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Key Benefits:
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Made for KingFisher™ Platforms
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Specifically optimized for SARS-COV2 (COVID-19) RNA isolation
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Can be adapted to a number of automated liquid handling stations
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Does not contain toxic organic solvents
Learn more details, protocols, and more in the tabs below.
From the nasal swabs used to test for COVID-19 (nasopharyngeal swabs) to bodily fluids, the HighPrep™ Viral RNA Isolation Kit isolates viral nucleic acids quickly and effectively. What’s more, we’ve designed this product to be versatile and efficient, so it works with manual workflows as well as a majority of liquid handling workstations.
| Product information area | Optimized for COVID-19 KingFisher™ Platforms |
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| Extra Info Button | Not using KingFisher? Click Here |
This is a short overview of the protocol. For detailed protocol please refer to PRODUCT DOCUMENTS
1. Gently swirl LES I container, then pipette 50uL to each well.
2. Add 200uL of sample to each well and pipette mix 15 times.
3. Incubate for 10mins at 37C.
4. Add 10uL Pro K solution to 250uL of pretreated sample mix.
5. Invert binding bead mix gently to mix, then add 280uL to each sample in the sample plate.
6. Select the program MVP_2Wash_200_ Flex on the instrument.
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Features |
Specifications |
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Main functions |
Isolation of Viral RNA from various sample matrices |
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Starting material (Sample matrix) |
Whole blood, saliva, swabs, plasma, samples collected in Viral Transport Media, serum |
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Sample amount (Input) |
can be scaled up |
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Downstream application |
Real time quantitative PCR, Reverse Transcriptase PCR, PCR, NGS |
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Process method |
Manual or automation |
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Instrument |
Adaptable to most nucleic acid purification instruments but a ready-made script for Kingfisher Flex is available upon request |
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Purification method |
Magnetic bead-based technology |
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Purification time |
23 min on Kingfisher Flex Instrument |
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